Cloning and sequencing of porcine LH-hCG receptor cDNA: variants lacking transmembrane domain

Complementary DNA clones, encoding the LH-hCG (luteinizing hormone-human choriogonadotropic hormone) receptor were isolated by screening a lambda gt11 library with monoclonal antibodies. The primary structure of the protein was deduced from the DNA sequence analysis; the protein contains 696 amino a...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1989-08, Vol.245 (4917), p.525-528
Main Authors: Loosfelt, H. (Institut National de la Sante et de la Recherche Medicale Unite, Kremlin Bicetre, France), Misrahi, M, Atger, M, Salesse, R, Vu Hai-Luv Thi, M, Jolivet, A, Guiochon-Mantel, A, Sar, S, Jallal, B, Garnier, J
Format: Article
Language:English
Subjects:
ADN
Amino Acid Sequence
Amino acids
Animals
ARN MENSAJERO
ARN MESSAGER
Base Sequence
Biological and medical sciences
Cell Membrane - metabolism
Cellular Biology
CERDO
chorionic gonadotropin
Cloning
Cloning, Molecular
Complementary DNA
COMPOSICION QUIMICA
COMPOSITION CHIMIQUE
COS cells
DNA
DNA - genetics
EXPRESION GENICA
EXPRESSION DES GENES
Female
Fundamental and applied biological sciences. Psychology
Generally accepted auditing standards
genes
Genes. Genome
GONADOTROPHINE
GONADOTROPINAS
GTP-Binding Proteins - metabolism
Hormones
LH receptors
Libraries
Life Sciences
Luteinizing hormone
Male
Messenger RNA
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Mutation
Nucleic Acid Hybridization
OVAIRE
OVARIOS
Ovary - analysis
pigs
PORCIN
Protein Sorting Signals - genetics
Proteins
RECEPTEUR D'HORMONE
RECEPTORES DE HORMONAS
Receptors
Receptors, LH - genetics
Receptors, LH - metabolism
RNA, Messenger - analysis
RNA, Messenger - genetics
SECUENCIA NUCLEICA
Sequence Homology, Nucleic Acid
SEQUENCE NUCLEIQUE
Swine
Testes
TESTICULE
TESTICULOS
Testis - analysis
Tissue Distribution
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Summary:Complementary DNA clones, encoding the LH-hCG (luteinizing hormone-human choriogonadotropic hormone) receptor were isolated by screening a lambda gt11 library with monoclonal antibodies. The primary structure of the protein was deduced from the DNA sequence analysis; the protein contains 696 amino acids with a putative signal peptide of 27 amino acids. Hydropathy analysis suggests the existence of seven transmembrane domains that show homology with the corresponding regions of other G protein-coupled receptors. Three other types of clones corresponding to shorter proteins were observed, in which the putative transmembrane domain was absent. These probably arose through alternative splicing. RNA blot analysis showed similar patterns in testis and ovary with a major RNA of 4700 nucleotides and several minor species. The messenger RNA was expressed in COS-7 cells, yielding a protein that bound hCG with the same affinity as the testicular receptor
ISSN:0036-8075
1095-9203
DOI:10.1126/science.2502844