Polyphasic study of Chryseobacterium strains isolated from diseased aquatic animals

Members of most Chryseobacterium species occur in aquatic environments or food products, while strains of some other species are pathogenic to humans and animals. A collection of 52 Chryseobacterium sp. strains isolated from diseased fish, one frog isolate and 22 reference strains were included in a...

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Bibliographic Details
Published in:Systematic and applied microbiology 2005-09, Vol.28 (7), p.640-660
Main Authors: Bernardet, J.-F., Vancanneyt, M., Matte-Tailliez, O., Grisez, L., Tailliez, P., Bizet, C., Nowakowski, M., Kerouault, B., Swings, J.
Format: Article
Language:English
Subjects:
16S rRNA gene sequence
Animal aquaculture
Animal productions
Animals
Anura
Anura - microbiology
bacterial infections
bacterial proteins
Bacterial Typing Techniques
Bacteriology
Biological and medical sciences
Chryseobacterium
Chryseobacterium - classification
Chryseobacterium - isolation & purification
Chryseobacterium - physiology
DNA, Bacterial - chemistry
Electrophoresis, Polyacrylamide Gel
Elizabethkingia
Fatty acid analysis
Fatty Acids - analysis
Fish disease
fish diseases
Fish Diseases - microbiology
Fishes
Flavobacteriaceae Infections - microbiology
Flavobacteriaceae Infections - veterinary
Frog disease
frogs
Fundamental and applied biological sciences. Psychology
Genes, Bacterial
Genes, rRNA
Life Sciences
Microbiology
Microbiology and Parasitology
molecular sequence data
molecular systematics
nucleotide sequences
Phenotype
Phenotypic characteristics
phenotypic variation
Phylogeny
Pisciculture
Polyphasic taxonomy
Random Amplified Polymorphic DNA Technique
Randomly amplified polymorphic DNA
ribosomal RNA
RNA, Ribosomal, 16S - genetics
SDS-PAGE
sequence analysis
Systematics
Vertebrate aquaculture
Whole-cell protein profiles
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Description
Summary:Members of most Chryseobacterium species occur in aquatic environments or food products, while strains of some other species are pathogenic to humans and animals. A collection of 52 Chryseobacterium sp. strains isolated from diseased fish, one frog isolate and 22 reference strains were included in a polyphasic taxonomy study. Fourteen clusters of strains were delineated following the comparison of whole-cell protein profiles. Most of these clusters were confirmed when the phenotypic and RAPD profiles and the 16S rRNA gene sequences were compared. Fatty acid composition helped differentiate the Chryseobacterium strains from members of related genera. None of the fish isolates could be allocated to the two species previously reported from fish but two isolates belonged to C. joostei, while the frog isolate was identified as Elizabethkingia meningoseptica, a human pathogen previously included in the genus Chryseobacterium. Three clusters grouping from 3 to 13 isolates will probably constitute the core of new Chryseobacterium species but all other isolates occupied separate or uncertain positions in the genus. This study further demonstrated the overall high similarity displayed by most Chryseobacterium strains whatever the technique used and the resulting difficulty in delineating new species in the genus. Members of this bacterial group should be considered potential emergent pathogens in various fish and frog species, farming conditions and geographical areas.
ISSN:0723-2020
1618-0984
DOI:10.1016/j.syapm.2005.03.016