Lipoxygenase-mediated production of fatty acid hydroperoxides is a specific signature of the hypersensitive reaction in plants

Lipoxygenase (LOX)-dependent massive production of (9 S) fatty acid hydroperoxides was previously observed in cryptogein-elicited tobacco leaves and proposed as being an actor of cell death during the hypersensitive reaction (HR). In this work, we have further investigated the occurrence of this met...

Full description

Saved in:
Bibliographic Details
Published in:Plant physiology and biochemistry 2002-06, Vol.40 (6), p.633-639
Main Authors: Montillet, Jean-Luc, Agnel, Jean-Pierre, Ponchet, Michel, Vailleau, Fabienne, Roby, Dominique, Triantaphylidès, Christian
Format: Article
Language:English
Subjects:
ARABIDOPSIS THALIANA
Bacterial plant pathogens
Biochemistry, Molecular Biology
Biological and medical sciences
DEFENCE MECHANISMS
ESTRES
Fundamental and applied biological sciences. Psychology
Generalities. Techniques. Transmission, epidemiology, ecology. Antibacterial substances, control
HOST PARASITE RELATIONS
Hypersensitive reaction
Life Sciences
Lipid hydroperoxide
LIPOXIGENASA
LIPOXYGENASE
MECANISME DE DEFENSE
MECANISMOS DE DEFENSA
Metabolism
Metabolism. Physicochemical requirements
OXIDACION
OXIDATION
OXYDATION
Oxylipins
Phytopathology. Animal pests. Plant and forest protection
Plant physiology and development
RELACIONES HUESPED PARASITO
RELATION HOTE PARASITE
STRESS
Tobacco
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Lipoxygenase (LOX)-dependent massive production of (9 S) fatty acid hydroperoxides was previously observed in cryptogein-elicited tobacco leaves and proposed as being an actor of cell death during the hypersensitive reaction (HR). In this work, we have further investigated the occurrence of this metabolism for biotic, compatible and incompatible interactions in tobacco, and also in Arabidopsis thaliana. Our methodology, based on metabolite analysis (isomer distribution and chirality), is sufficient to assess for the induction of a LOX metabolism. In both plants, a (13 S) oxylipin metabolism is initially operating in control leaves. In tobacco, the (9 S) LOX-dependent oxylipin metabolism was shown to be induced by tobacco mosaic virus and an avirulent bacterial strain of Ralstonia solanacearum. In Arabidopsis, accumulation of the oxylipin metabolites in leaves was also observed in response to harpin, and during different incompatible interactions. However, in the latter model, the metabolites are mainly (13 S), suggesting the involvement of a specific (13 S) LOX. In all cases studied so far, accumulation of the oxylipin metabolites is correlated with HR cell death and is not observed during compatible interactions. In many plant species, LOX transcript accumulation precedes, and LOX activity correlates, the induction of the HR symptoms. Thus, accumulation of the oxylipin metabolites can be considered as a marker of HR cell death in plant–pathogen interactions. Both 9 or 13 oxylipin metabolism can be apparently activated, depending on the plant species.
ISSN:0981-9428
1873-2690
DOI:10.1016/S0981-9428(02)01402-X