Use of Glutamine and Low Density Lipoproteins Isolated from Egg Yolk to Improve Buck Semen Freezing

To improve the results obtained with a reference cryopreservation extender (control extender: Triladyl® + 20% (v/v) egg yolk + 6.4% (v/v) glycerol) for freezing caprine semen, glutamine was added to 18 split ejaculates at concentrations of 0, 20, 40, 80 and 120 m m (experiment 1). In experiment 2, g...

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Published in:Reproduction in domestic animals 2008-08, Vol.43 (4), p.429-436
Main Authors: Ali Al Ahmad, MZ, Chatagnon, G, Amirat-Briand, L, Moussa, M, Tainturier, D, Anton, M, Fieni, F
Format: Article
Language:English
Subjects:
Acrosome - drug effects
Acrosome - physiology
Acrosome Reaction - drug effects
Acrosome Reaction - physiology
Agricultural sciences
Animal reproduction
Animals
Biological and medical sciences
Body fluids
Cryogenic engineering
Cryopreservation - methods
Cryopreservation - veterinary
Cryoprotective Agents - pharmacology
Dose-Response Relationship, Drug
Egg Yolk - chemistry
Fundamental and applied biological sciences. Psychology
Glutamine - pharmacology
Goats
Goats - physiology
Life Sciences
Lipoproteins, LDL - pharmacology
Low density lipoprotein
Male
Males
Mammalian reproduction. General aspects
Semen - cytology
Semen - drug effects
Semen - physiology
Semen Preservation - methods
Semen Preservation - veterinary
Sperm Motility - drug effects
Sperm Motility - physiology
Spermatozoa - drug effects
Spermatozoa - physiology
Vertebrates: reproduction
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Summary:To improve the results obtained with a reference cryopreservation extender (control extender: Triladyl® + 20% (v/v) egg yolk + 6.4% (v/v) glycerol) for freezing caprine semen, glutamine was added to 18 split ejaculates at concentrations of 0, 20, 40, 80 and 120 m m (experiment 1). In experiment 2, glutamine was added to 18 split ejaculates at concentrations of 20, 25, 30, 35 and 40 m m. In the third experiment, the egg yolk was replaced with the low-density lipoprotein (LDL) fraction of egg yolk. The quality of frozen then thawed spermatozoa in each extender was compared using computer-assisted semen analysis. In experiment 1, glutamine at concentrations of 20 m m and 40 m m significantly improved sperm motility compared with the control extender. However, at 120 m m, a significant decrease in motility and velocity was observed. In experiment 2, motility, curvilinear velocity and amplitude of lateral head displacement (ALH) were improved in glutamine at 25 m m compared with the control. In experiment 3, 8% LDL and 25 m m glutamine significantly improved sperm motility, straight line velocity and ALH. In the fourth experiment, the quality of the previously defined freezing extender (Triladyl® + 8% (v/v) LDL + 25 m m glutamine + 6.4% (v/v) glycerol) was tested by comparing acrosome, tail membrane, plasma membrane and DNA integrity in 18 split ejaculates of frozen then thawed spermatozoa with spermatozoa that had been frozen then thawed in the control extender, and with spermatozoa from fresh, unfrozen sperm. The percentage of spermatozoa with intact acrosomes and tail membranes was significantly higher with the newly defined extender than that observed with the control extender. There was no significant difference in the percentage of spermatozoa with intact DNA between the frozen and fresh semen.
ISSN:0936-6768
1439-0531
DOI:10.1111/j.1439-0531.2007.00930.x