Transcriptome analysis of root-knot nematode functions induced in the early stages of parasitism

Root-knot nematodes of the genus Meloidogyne are obligate biotrophic parasites able to infest > 2000 plant species. The nematode effectors responsible for disease development are involved in the adaptation of the parasite to its host environment and host response modulation. Here, the differences...

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Bibliographic Details
Published in:The New phytologist 2007-01, Vol.176 (2), p.426-436
Main Authors: Dubreuil, G, Magliano, M, Deleury, E, Abad, P, Rosso, M.N
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Complementary DNA
Double stranded RNA
Gene Expression Profiling
Gene Expression Regulation
Gene Library
Genes
Giant cells
Glutathione Transferase - chemistry
Glutathione Transferase - metabolism
Glutathione Transferase - physiology
Helminth Proteins - chemistry
Helminth Proteins - metabolism
Helminth Proteins - physiology
Host-Parasite Interactions - genetics
Libraries
Life Sciences
Molecular Sequence Data
nematode parasitism gene
nematode secretion
Nematodes
Nucleic Acid Hybridization
Parasite hosts
Phytopathology and phytopharmacy
plant defence response
Plant parasitic nematodes
plant-microbe interaction
Plants
Plants - parasitology
Polymerase Chain Reaction
RNA, Messenger - metabolism
Root knot nematodes
Sequence Alignment
suppression subtractive hybridization
Tylenchoidea - genetics
Tylenchoidea - growth & development
Tylenchoidea - metabolism
Vegetal Biology
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Summary:Root-knot nematodes of the genus Meloidogyne are obligate biotrophic parasites able to infest > 2000 plant species. The nematode effectors responsible for disease development are involved in the adaptation of the parasite to its host environment and host response modulation. Here, the differences between the transcriptomes of preparasitic exophytic second-stage juveniles (J2) and parasitic endophytic third-stage juveniles (J3) of Meloidogyne incognita were investigated. Genes up-regulated at the endophytic stage were isolated by suppression subtractive hybridization and validated by dot blots and real-time quantitative polymerase chain reaction (PCR). Up-regulation was demonstrated for genes involved in detoxification and protein degradation, for a gene encoding a putative secreted protein and for genes of unknown function. Transcripts of the glutathione S-transferase gene Mi-gsts-1 were 27 times more abundant in J3 than in J2. The observed Mi-gsts-1 expression in the oesophageal secretory glands and the results of functional analyses based on RNA interference suggest that glutathione S-transferases are secreted during parasitism and are required for completion of the nematode life cycle in its host. Secreted glutathione S-transferases may protect the parasite against reactive oxygen species or modulate the plant responses triggered by pathogen attack.
ISSN:0028-646X
1469-8137
DOI:10.1111/j.1469-8137.2007.02181.x