Temporal and spatial changes in cell wall composition in developing grains of wheat cv. Hereward

A combination of enzyme mapping, FT-IR microscopy and NMR spectroscopy was used to study temporal and spatial aspects of endosperm cell wall synthesis and deposition in developing grain of bread wheat cv. Hereward. This confirmed previous reports that changes in the proportions of the two major grou...

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Bibliographic Details
Published in:Planta 2010-08, Vol.232 (3), p.677-689
Main Authors: Toole, G. A, Le Gall, G, Colquhoun, I. J, Nemeth, C, Saulnier, L, Lovegrove, A, Pellny, T, Wilkinson, M. D, Freeman, J, Mitchell, R. A. C, Mills, E. N. C, Shewry, P. R
Format: Article
Language:English
Subjects:
Agriculture
arabinoxylan
Biological and medical sciences
Biomedical and Life Sciences
Cell wall
Cell Wall - chemistry
Cell wall components
Cell walls
Chromatography, High Pressure Liquid
Chromatography, Ion Exchange
Desiccation
Developmental biology
Dietary fibre
Ecology
Endosperm
Enzymes
Forestry
Fundamental and applied biological sciences. Psychology
Gene Expression Profiling
Genetics
Grain
Grains
Life Sciences
Magnetic Resonance Spectroscopy
Original Article
Pixels
Plant Sciences
Plants genetics
Polysaccharides
Principal Component Analysis
Principal components analysis
Ratios
RNA, Messenger - genetics
Saccharides
Spectroscopy, Fourier Transform Infrared
Triticum - chemistry
Triticum - genetics
Triticum - growth & development
Wheat
Wheat grain
β-Glucan
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Summary:A combination of enzyme mapping, FT-IR microscopy and NMR spectroscopy was used to study temporal and spatial aspects of endosperm cell wall synthesis and deposition in developing grain of bread wheat cv. Hereward. This confirmed previous reports that changes in the proportions of the two major groups of cell wall polysaccharides occur, with β-glucan accumulating earlier in development than arabinoxylan. Changes in the structure of the arabinoxylan occurred, with decreased proportions of disubstituted xylose residues and increased proportions of monosubstituted xylose residues. These are likely to result, at least in part, from arabinoxylan restructuring catalysed by enzymes such as arabinoxylan arabinofurano hydrolase and lead to changes in cell wall mechanical properties which may be required to withstand stresses during grain maturation and desiccation.
ISSN:0032-0935
1432-2048
DOI:10.1007/s00425-010-1199-5