TRB3 Inhibits the Transcriptional Activation of Stress-regulated Genes by a Negative Feedback on the ATF4 Pathway

The integrated stress response (ISR) is defined as a highly conserved response to several stresses that converge to the induction of the activating transcription factor 4 (ATF4). Because an uncontrolled response may have deleterious effects, cells have elaborated several negative feedback loops that...

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Published in:	The Journal of biological chemistry 2007-05, Vol.282 (21), p.15851-15861
Main Authors:	Jousse, Céline, Deval, Christiane, Maurin, Anne-Catherine, Parry, Laurent, Chérasse, Yoan, Chaveroux, Cédric, Lefloch, Renaud, Lenormand, Philippe, Bruhat, Alain, Fafournoux, Pierre
Format:	Article
Language:	eng
Subjects:	Activating Transcription Factor 4 - biosynthesis Activating Transcription Factor 4 - genetics Amino Acids - deficiency Animals Aspartate-Ammonia Ligase - biosynthesis Aspartate-Ammonia Ligase - genetics Biochemistry Biochemistry, Molecular Biology Cell Cycle Proteins - genetics Cell Cycle Proteins - metabolism Drosophila Gene Silencing HeLa Cells Humans Life Sciences Mice Models, Biological Protein-Serine-Threonine Kinases - genetics Protein-Serine-Threonine Kinases - metabolism Repressor Proteins - genetics Repressor Proteins - metabolism Stress, Physiological - genetics Stress, Physiological - metabolism Transcription Factor CHOP - biosynthesis Transcription Factor CHOP - genetics
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Summary:	The integrated stress response (ISR) is defined as a highly conserved response to several stresses that converge to the induction of the activating transcription factor 4 (ATF4). Because an uncontrolled response may have deleterious effects, cells have elaborated several negative feedback loops that attenuate the ISR. In the present study, we describe how induction of the human homolog of Drosophila tribbles (TRB3) attenuates the ISR by a negative feedback mechanism. To investigate the role of TRB3 in the control of the ISR, we used the regulation of gene expression by amino acid limitation as a model. The enhanced production of ATF4 upon amino acid starvation results in the induction of a large number of target genes like CHOP (CAAT/enhancer-binding protein-homologous protein), asparagine synthetase (ASNS), or TRB3. We demonstrate that TRB3 overexpression inhibits the transcriptional induction of CHOP and ASNS whereas TRB3 silencing induces the expression of these genes both under normal and stressed conditions. In addition, transcriptional profiling experiments show that TRB3 affects the expression of many ISR-regulated genes. Our results also suggest that TRB3 and ATF4 belong to the same protein complex bound to the sequence involved in the ATF4-dependent regulation of gene expression by amino acid limitation. Collectively, our data identify TRB3 as a negative feedback regulator of the ATF4-dependent transcription and participates to the fine regulation of the ISR.
ISSN:	0021-9258 1083-351X