Identification of phenotypic discriminating markers for intervertebral disc cells and articular chondrocytes

Objective. The present study was conducted to improve our knowledge of intervertebral disc (IVD) cell biology by comparing the phenotype of nucleus pulposus (NP) and annulus fibrosus (AF) cells with that of articular chondrocytes (ACs). Methods. Rabbit cells from NP and AF were isolated and their ph...

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Published in:Rheumatology 2009-11, Vol.48 (11), p.1447-1450
Main Authors: Clouet, Johann, Grimandi, Gaël, Pot-Vaucel, Marianne, Masson, Martial, Fellah, Hakim B., Guigand, Lydie, Cherel, Yan, Bord, Eric, Rannou, François, Weiss, Pierre, Guicheux, Jérôme, Vinatier, Claire
Format: Article
Language:English
Subjects:
Animals
Annulus fibrosus
Biological and medical sciences
Biomarkers - metabolism
Cartilage, Articular - cytology
Cartilage, Articular - metabolism
Chondrocyte
Chondrocytes - cytology
Chondrocytes - metabolism
Diseases of the osteoarticular system
Extracellular Matrix Proteins - biosynthesis
Extracellular Matrix Proteins - genetics
Intervertebral disc
Intervertebral Disc - cytology
Intervertebral Disc - metabolism
Life Sciences
Medical sciences
Nucleus pulposus
Phenotype
Rabbits
Reverse Transcriptase Polymerase Chain Reaction - methods
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Summary:Objective. The present study was conducted to improve our knowledge of intervertebral disc (IVD) cell biology by comparing the phenotype of nucleus pulposus (NP) and annulus fibrosus (AF) cells with that of articular chondrocytes (ACs). Methods. Rabbit cells from NP and AF were isolated and their phenotype was compared with that of AC by real-time PCR analysis of type I (COL1A1), II (COL2A1) and V (COL5A1) collagens, aggrecan transcript (AGC1), matrix Gla protein (MGP) and Htra serine peptidase 1 (Htra1). Results. Transcript analysis indicated that despite certain similarities, IVD cells exhibit distinct COL2A1/COL1A1 and COL2A1/AGC1 ratios as compared with AC. The expression pattern of COL5A1, MGP and Htra1 makes it possible to define a phenotypic signature for NP and AF cells. Conclusions. Our study shows that NP and AF cells exhibit a clearly distinguishable phenotype from that of AC. Type V collagen, MGP and HtrA1 could greatly help to discriminate among NP, AF and AC cells.
ISSN:1462-0324
1462-0332
1460-2172
DOI:10.1093/rheumatology/kep262