Partial characterization of two divergent variants of Grapevine leafroll-associated virus 4 [Vitis vinifera L.; Israel; Turkey]

Two non mechanically transmissible viruses with filamentous closterovirus-like particles were extracted and partially characterized from leafroll-affected grapevine accessions of cv Koussan from Turkey (Y253) and cv Koudsi from Israel (Y252), both from the reference collection of grapevine viruses a...

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Bibliographic Details
Published in:Journal of plant pathology 2006-07, Vol.88 (2), p.203-214
Main Authors: Saldarelli, P, Cornuet, P, Vigne, E, Talas, F, Bronnenkant, I, Dridi, A.M, Andret-Link, P, Boscia, D, Gungerli, P, Fuchs, M, Martelli, G.P
Format: Article
Language:English
Subjects:
AMINO ACID SEQUENCES
Amino acids
Antibodies
Antiserum
ARN
Closteroviridae
DISTANCE GENETIQUE
DISTANCIA GENETICA
distanza genetica
elisa
GENETIC DISTANCE
GENETIC VARIATION
GRAPEVINE LEAF ROLL VIRUS
Grapevine leafroll-associated virus 4
GRAPEVINES
http://www.fao.org/aos/agrovoc#c_15968
http://www.fao.org/aos/agrovoc#c_15975
http://www.fao.org/aos/agrovoc#c_27530
http://www.fao.org/aos/agrovoc#c_33358
http://www.fao.org/aos/agrovoc#c_3360
http://www.fao.org/aos/agrovoc#c_34079
http://www.fao.org/aos/agrovoc#c_37202
http://www.fao.org/aos/agrovoc#c_3791
http://www.fao.org/aos/agrovoc#c_3801
http://www.fao.org/aos/agrovoc#c_3807
http://www.fao.org/aos/agrovoc#c_3972
http://www.fao.org/aos/agrovoc#c_4810
http://www.fao.org/aos/agrovoc#c_6618
http://www.fao.org/aos/agrovoc#c_8013
http://www.fao.org/aos/agrovoc#c_8157
http://www.fao.org/aos/agrovoc#c_8283
IDENTIFICACION
IDENTIFICATION
identificazione
IMMUNE SERUM
IMMUNOLOGICAL TECHNIQUES
IMMUNSERUM
ISRAEL
israele
Life Sciences
microscopia
MICROSCOPIE
MICROSCOPY
pcr
Phylogenetics
Phytopathology
Phytopathology and phytopharmacy
Plant diseases
Reverse transcriptase polymerase chain reaction
rna
SECUENCIAS DE AMINOACIDOS
SEQUENCE D'ACIDES AMINES
sequenze aminoacidiche
siero immune
SUERO INMUNE
TECHNIQUE IMMUNOLOGIQUE
TECNICAS INMUNOLOGICAS
tecniche immunologiche
TEST ELISA
turchia
TURKEY
TURQUIA
TURQUIE
VARIACION GENETICA
VARIATION GENETIQUE
variazione genetica
VARIEDADES
VARIETE
VARIETIES
varietà
Vegetal Biology
VID
VIGNE
Viral diseases
VIRUS DEL ENROLLADO DE LA VID
virus dell'accartocciamento fogliare della vite
VIRUS ENROULEMENT DE LA VIGNE
Viruses
Vitaceae
viti
vitis vinifera
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Description
Summary:Two non mechanically transmissible viruses with filamentous closterovirus-like particles were extracted and partially characterized from leafroll-affected grapevine accessions of cv Koussan from Turkey (Y253) and cv Koudsi from Israel (Y252), both from the reference collection of grapevine viruses and virus-like diseases at the Institut National de la Recherche Agronomique (INRA) in Colmar, France. These viruses, denoted Y253-TK and Y252-IL, were independently investigated in France (INRA-Colmar) and Italy (DPPMA). Y253-TK did not react in DAS-ELISA or RT-PCR assays that would have detected any of the Grapevine leafroll-associated viruses (GLRaVs) described so far; Y252-IL did give weak and inconsistent positive reactions in IEM only with an antiserum to strain LR106 of Grapevine leafroll-associated virus 4 (GLRaV-4). A polyclonal antiserum to isolate Y252-IL decorated the homologous particles and, those of Y253-TK and, at lower dilution, particles of isolate GLRaV-4 LR106. A segment of the heat shock protein p70 homologue (HSP70h) gene, was amplified by RT-PCR of RNA from denatured purified particles of Y253-TK by using a set of degenerate primers. The 183 amino acid polypeptide deduced from the 549 bp HSP70h gene fragment showed a high degree of identity with the cognate genes of GLRaV-4 (95%), GLRaV-5 (91%), GLRaV-6 (84%), and GLRaV-9 (90%), but low identity with those of other GLRaVs (33-35%). The HSP70h of isolates Y252-IL and LR106 showed 95% amino acid identity. The coat protein sequence of isolates Y253-TK showed 99 and 94% amino acid identity with those of isolates Y252-IL and LR106, respectively, with changes concentrated in the N-terminus, a feature that might have a bearing on the reaction with their heterologous antisera. Based on serological and molecular data, it was concluded that Y253-TK and Y252-IL are distinct, though similar, isolates of GLRaV-4. In two independent surveys in which 110 and 320 grapevine accessions from germplasm collections at Colmar and Bari, respectively, were examined by DAS-ELISA, the antisera As-Y253-TK and As-Y252-IL cross-reacted with the homologous and heterologous viruses. Whereas As-Y253-TK did not react with any of the other accessions from the French collection tested, As-Y252-IL recognized a virus present in a total of eight grapevine accessions from the Mediterranean area, grown in the Italian germplasm collection.
ISSN:1125-4653
2239-7264