Acetaldehyde addition throughout the growth phase alleviates the phenotypic effect of zinc deficiency in Saccharomyces cerevisiae

During experiments to determine the effects of exogenously added acetaldehyde on pure cultures of various yeast strains, we discovered that an early acetaldehyde perfusion during the growth phase allowed several yeasts to partially overcome the phenotypic effects of zinc depletion during alcoholic f...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2008, Vol.77 (5), p.1093-1109
Main Authors: Cheraiti, Naoufel, Sauvage, François-Xavier, Salmon, Jean-Michel
Format: Article
Language:English
Subjects:
acetaldehyde
Acetaldehyde - metabolism
Acids
Alcohol Dehydrogenase - biosynthesis
alcoholic fermentation
anaerobiosis
Applied Microbial and Cell Physiology
Biological and medical sciences
Biotechnology
Electrophoresis, Gel, Two-Dimensional
Ethanol
Ethanol - metabolism
Fermentation
Fundamental and applied biological sciences. Psychology
Gene Expression Profiling
Genomes
Genotype & phenotype
Glucose
Laboratories
Life Sciences
Metabolism
Microbial Genetics and Genomics
Microbiology
Microbiology and Parasitology
NAD - metabolism
Oligonucleotide Array Sequence Analysis
Proteome - analysis
Saccharomyces cerevisiae
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins - biosynthesis
Studies
Yeast
yeasts
Zinc
Zinc - metabolism
Zinc depletion
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Summary:During experiments to determine the effects of exogenously added acetaldehyde on pure cultures of various yeast strains, we discovered that an early acetaldehyde perfusion during the growth phase allowed several yeasts to partially overcome the phenotypic effects of zinc depletion during alcoholic fermentation. We, therefore, performed genome-wide expression and proteomic analysis on an industrial Saccharomyces cerevisiae yeast strain (VL1) growing in zinc-replete or zinc-depleted conditions in the presence of perfused acetaldehyde to identify molecular markers of this effect. Zinc depletion severely affects ethanol production and therefore nicotinamide adenine dinucleotide (NAD) regeneration, although we observed partial compensation by the upregulation of the poorly efficient Fe-dependent Adh4p in our conditions. A coordinate metabolic response was indeed observed in response to the early acetaldehyde perfusion, and particularly of the lower part of glycolysis, leading to the cellular replenishment of NAD cofactor. These various findings suggest that acetaldehyde exchange between strains may inhibit the growth of some yeast strains while encouraging the growth of others. This phenomenon could be particularly important for understanding the ecology of colonization of complex fermentation media by S. cerevisiae after elimination of non-Saccharomyces yeasts.
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-007-1234-z