Synthesis of biodiesel in column fixed-bed bioreactor using the fermented solid produced by Burkholderia cepacia LTEB11

We produced a lipase from Burkholderia cepacia in solid-state fermentation and used it to catalyze the synthesis of biodiesel in a fixed-bed reactor. In the solid-state fermentation step, a 50:50 (by mass) mixture of sugarcane bagasse and sunflower seed meal gave 234 units of pNPP-hydrolyzing activi...

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Bibliographic Details
Published in:Process biochemistry (1991) 2010-08, Vol.45 (8), p.1348-1354
Main Authors: Salum, Thaís Fabiana Chan, Villeneuve, Pierre, Barea, Bruno, Yamamoto, Carlos Itsuo, Côcco, Lílian Cristina, Mitchell, David Alexander, Krieger, Nadia
Format: Article
Language:English
Subjects:
Biochemistry, Molecular Biology
Biodiesel
Burkholderia
Burkholderia cepacia
Column packings
Fermentation
Fixed-bed reactor
Helianthus
Life Sciences
Lipase
Solid-state fermentation
Sunflowers
Synthesis
Transesterification
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Summary:We produced a lipase from Burkholderia cepacia in solid-state fermentation and used it to catalyze the synthesis of biodiesel in a fixed-bed reactor. In the solid-state fermentation step, a 50:50 (by mass) mixture of sugarcane bagasse and sunflower seed meal gave 234 units of pNPP-hydrolyzing activity per gram of dry solids at 96 h. This fermented solid was lyophilized and delipidated, packed into a column and used to catalyze the synthesis of biodiesel through the ethanolysis of soybean oil in a medium free of co-solvents, with the reaction mixture being continuously circulated through the column. The best conversion was 95% after 46 h, which was obtained at 50 °C, with an alcohol:oil molar ratio of 3:1, alcohol addition in two steps and the addition of 1% of (m/m) water to the reaction medium. This system has potential to decrease the costs of enzyme-catalyzed transesterification reactions.
ISSN:1359-5113
1873-3298
DOI:10.1016/j.procbio.2010.05.004