accurate real-time PCR test for the detection and quantification of cauliflower mosaïc virus (CaMV): applicable in GMO screening

Due to its very large use in the first generation of genetically modified organisms (GMO), the 35S promoter derived from the cauliflower mosaic virus (CaMV) is the most used PCR target for screening tests in GMO routine analysis, before any identification and quantification of GMOs. Accordingly, a s...

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Bibliographic Details
Published in:European food research & technology 2008-07, Vol.227 (3), p.789-798
Main Authors: Chaouachi, Maher, Fortabat, Marie Noelle, Geldreich, Angèle, Yot, Pierre, Kerlan, Camille, Kebdani, Naïma, Audeon, Colette, Romaniuk, Marcel, Bertheau, Yves
Format: Article
Language:eng ; ger
Subjects:
Agriculture
Analytical Chemistry
Biological and medical sciences
Biotechnology
Cauliflower mosaic virus
Chemistry
Chemistry and Materials Science
Food
Food engineering
Food industries
Food Science
Forestry
Fundamental and applied biological sciences. Psychology
General aspects
Genomes
Laboratories
Life Sciences
Methods of analysis, processing and quality control, regulation, standards
Organisms
Original Paper
Plant biology
Proteins
Real time
Studies
Viruses
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Summary:Due to its very large use in the first generation of genetically modified organisms (GMO), the 35S promoter derived from the cauliflower mosaic virus (CaMV) is the most used PCR target for screening tests in GMO routine analysis, before any identification and quantification of GMOs. Accordingly, a specific detection of the virus donor organism is required to avoid false positives. A new qualitative and quantitative method based on real time polymerase chain reaction (PCR) techniques was developed for the detection and quantification of CaMV. The region targeted was an internal part of a qualitative test previously published using the ORFIII of the CaMV genome. It codes for a protein (P3) responsible for the pathogen infectivity and not in the ORFIV coding for a coat protein (P4), which can be used for GMO constructions. In this paper, we show the high reliability of the PCR test both in simplex and duplex (with P35S of the CaMV). This test shows high specificity and sensitivity (LODa
ISSN:1438-2377
1438-2385
DOI:10.1007/s00217-007-0787-5