Identification and characterization of nuclear microsatellites in Mediterranean cedars (Cedrus sp.)

We developed primers for the amplification of six polymorphic nuclear microsatellites in Mediterranean Cedrus taxa. Microsatellites originated from two Cedrus atlantica genomic libraries enriched for TC (four markers) and TG (two markers) motifs. No distortion from Mendelian segregation was observed...

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Bibliographic Details
Published in:Molecular ecology notes 2006-09, Vol.6 (3), p.840-842
Main Authors: CHAIB, J, DANAN, S, JOUAUD, B, HAGEN, L.S, LEFEVRE, F, FADY, B
Format: Article
Language:English
Subjects:
Cedrus
DNA library
Ecology, environment
heterozygosity
Life Sciences
microsatellite
primer
segregation
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Summary:We developed primers for the amplification of six polymorphic nuclear microsatellites in Mediterranean Cedrus taxa. Microsatellites originated from two Cedrus atlantica genomic libraries enriched for TC (four markers) and TG (two markers) motifs. No distortion from Mendelian segregation was observed. There were up to eight alleles per locus and mean expected heterozygosity was 0.75. Four microsatellites also amplified in the Himalayan cedar. These markers should be helpful for species identification, diversity studies, parentage analysis and genome mapping and to monitor biodiversity in endangered Mediterranean Cedrus forests.
ISSN:1471-8278
1471-8286
DOI:10.1111/j.1471-8286.2006.01364.x