Enhanced Polar Auxin Transport in Tomato Polycotyledon Mutant Seems to be Related to Glutathione Levels

Glutathione-dependent root growth in Arabidopsis is linked to polar auxin transport (PAT). Arabidopsis mutants with reduced glutathione (GSH) levels also show reduced PAT. To gain an insight into the relationship between PAT and GSH level, we analyzed tomato polycotyledon mutant, pct1-2, which has e...

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Bibliographic Details
Published in:Journal of plant growth regulation 2021-04, Vol.40 (2), p.761-773
Main Authors: Nongmaithem, Sapana, Ponukumatla, Rachana, Sreelakshmi, Yellamaraju, Frasse, Pierre, Bouzayen, Mondher, Sharma, Rameshwar
Format: Article
Language:English
Subjects:
Acids
Agriculture
Arabidopsis
Benzoic acid
Biochemistry, Molecular Biology
Biomedical and Life Sciences
Buthionine sulfoximine
DNA microarrays
Elongation
Gene expression
Glutathione
Inhibitors
Life Sciences
Metabolism
Mutants
p-Hydroxyphenylacetic acid
Phenotypes
Pin1 protein
Plant Anatomy/Development
Plant Physiology
Plant Sciences
Seedlings
Tips
Tomatoes
Vegetal Biology
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Summary:Glutathione-dependent root growth in Arabidopsis is linked to polar auxin transport (PAT). Arabidopsis mutants with reduced glutathione (GSH) levels also show reduced PAT. To gain an insight into the relationship between PAT and GSH level, we analyzed tomato polycotyledon mutant, pct1-2, which has enhanced PAT. Microarray analysis of gene expression in pct1-2 mutant revealed underexpression of several genes related to glutamate and glutathione metabolism. In consonance with microarray analysis, enzymatic as well as in vivo assay revealed higher glutathione levels in the early phase of pct1-2 seedling growth than WT. The inhibition of auxin transport by 2,3,5-triiodobenzoic acid (TIBA) reduced both GSH level and PIN1 expression in pct1-2 root tips . The reduction of in vivo GSH accumulation in pct1-2 root tips by buthionine sulfoximine (BSO) stimulated elongation of the short root of pct1-2 mutant akin to TIBA. The rescue of the short root phenotype of the pct1-2 mutant was restricted to TIBA and BSO. The other auxin transport inhibitors 1- N -naphthylphthalamic acid (NPA), 2-[4-(diethylamino)-2-hydroxybenzoyl] benzoic acid (BUM), 3-chloro-4-hydroxyphenylacetic acid (CHPAA), brefeldin and gravacin inhibited root elongation in both WT and pct1-2 mutant. Our results indicate a relationship between PAT and GSH levels in tomato akin to Arabidopsis. Our work also highlights that TIBA rescues the short root phenotype of the pct1-2 mutant by acting on a PAT component distinct from the site of action of other PAT inhibitors.
ISSN:0721-7595
1435-8107
DOI:10.1007/s00344-020-10139-8