Development, mapping and transferability of Fragaria EST-SSRs within the Rosodae supertribe

With 1 figure and 3 tables ABSTRACT: The Rosodae supertribe is composed of economically important species (strawberry, rose and raspberry). In order to extend the repertoire of markers for optimizing genetic mapping and synteny within this supertribe, we developed 20 functional markers from two Frag...

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Bibliographic Details
Published in:Plant breeding 2011-04, Vol.130 (2), p.248-255
Main Authors: Rousseau-Gueutin, M, Richard, L, Le Dantec, L, Caron, H, Denoyes-Rothan, B
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Biological and medical sciences
cDNA libraries
chromosome mapping
Classical and quantitative genetics. Population genetics. Molecular genetics
complementary DNA
diploidy
Duchesnea indica
Fragaria
functional markers
Fundamental and applied biological sciences. Psychology
Generalities. Genetics. Plant material
Genetics and breeding of economic plants
genic microsatellites
Life Sciences
marker transferability
marker-assisted selection
Molecular genetics
polymorphism
Potentilla
Rosa
Rosodae
strawberries
strawberry
Vegetal Biology
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Summary:With 1 figure and 3 tables ABSTRACT: The Rosodae supertribe is composed of economically important species (strawberry, rose and raspberry). In order to extend the repertoire of markers for optimizing genetic mapping and synteny within this supertribe, we developed 20 functional markers from two Fragaria cDNA libraries enriched in AG/AC microsatellite motifs. We tested their transferability within the Rosodae supertribe, including two reference diploid and octoploid strawberry segregating populations. Out of these 20 functional markers, 70% revealed polymorphism in both diploid and octoploid Fragaria segregating populations and were mapped on the diploid genetic map to provide useful information for further marker-assisted selection. These 20 EST-SSRs were also tested in 17 wild Fragaria species and displayed a high cross-species transferability (mean of 95%), providing new markers for further diversity studies within the genus. These markers were also transferable to the phylogenetically close Potentilla genus, whereas their transferability was lower for the polyploid Duchesnea indica species and for the more distant Rosa genus. Their use for genetic mapping within the Rosodae will further permit a comparison of genetic information among the different genera.
ISSN:0179-9541
1439-0523
DOI:10.1111/j.1439-0523.2010.01785.x