Dietary n-3 PUFA affect lipid metabolism and tissue function-related genes in bovine muscle

Gene expression profiles of bovine longissimus muscle as affected by dietary n-3 v.n-6 fatty acid (FA) intervention were analysed by microarray pre-screening of >3000 muscle biology/meat quality-related genes as well as subsequent quantitative RT-PCR gene expression validation of genes encoding l...

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Bibliographic Details
Published in:	British journal of nutrition 2012-09, Vol.108 (5), p.858-863
Main Authors:	Hiller, Beate, Hocquette, Jean-Francois, Cassar-Malek, Isabelle, Nuernberg, Gerd, Nuernberg, Karin
Format:	Article
Language:	eng
Subjects:	Animal biology Animals Base Sequence Biological and medical sciences Cattle DNA Primers Fatty acids Fatty Acids, Omega-3 - administration & dosage Fatty Acids, Omega-3 - pharmacology Feeding. Feeding behavior Fundamental and applied biological sciences. Psychology Gene expression Gene Expression Profiling Life Sciences Lipids Male Metabolism Muscle, Skeletal - drug effects Muscle, Skeletal - metabolism Muscular system Oligonucleotide Array Sequence Analysis Real-Time Polymerase Chain Reaction Vertebrates: anatomy and physiology, studies on body, several organs or systems
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Summary:	Gene expression profiles of bovine longissimus muscle as affected by dietary n-3 v.n-6 fatty acid (FA) intervention were analysed by microarray pre-screening of >3000 muscle biology/meat quality-related genes as well as subsequent quantitative RT-PCR gene expression validation of genes encoding lipogenesis-related transcription factors (CCAAT/enhancer-binding protein β, sterol regulatory element-binding transcription factor 1), key-lipogenic enzymes (acetyl-CoA carboxylase α (ACACA), fatty acid synthase (FASN), stearoyl-CoA desaturase (SCD)), lipid storage-associated proteins (adipose differentiation-related protein (ADFP)) and muscle biology-related proteins (cholinergic receptor, nicotinic, α1, farnesyl diphosphate farnesyl transferase 1, sema domain 3C (SEMA3C)). Down-regulation of ACACA (P = 0·00), FASN (P = 0·09) and SCD (P = 0·02) gene expression upon an n-3 FA intervention directly corresponded to reduced SFA, MUFA and total FA concentrations in longissimus muscle, whereas changes in ADFP (P = 0·00) and SEMA3C (P = 0·05) gene expression indicated improved muscle function via enhanced energy metabolism, vasculogenesis, innervation and mediator synthesis. The present study highlights the significance of dietary n-3 FA intervention on muscle development, maintenance and function, which are relevant for meat quality tailoring of bovine tissues and modulating animal production-relevant physiological processes.
ISSN:	0007-1145 1475-2662