Metabolic effects in mice of cream formulation: Addition of both thickener and emulsifier does not alter lipid metabolism but modulates mucus cells and intestinal endoplasmic reticulum stress

Additives stabilize or improve the organoleptic or functional properties (or both) of many dairy products including whipping cream. Their influence on the metabolic effect of dairy cream is scarcely known. We tested the hypothesis that added emulsifier (lactic acid esters of mono- and diglycerides;...

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Bibliographic Details
Published in:Journal of dairy science 2018-12, Vol.101 (12), p.10649-10663
Main Authors: Milard, M., Laugerette, F., Bugeat, S., Plaisancié, P., Létisse, M., Meugnier, E., Loizon, E., Durand, A., Buisson, C., Géloën, A., Serieye, S., Michalski, M.-C.
Format: Article
Language:English
Subjects:
Animals
carrageenan
Cattle
cow milk
dairy matrix
Dairy Products - analysis
Duodenum - metabolism
Emulsifying Agents - analysis
Emulsifying Agents - metabolism
Endoplasmic Reticulum Stress
Fatty Acids, Nonesterified - blood
Food Additives - analysis
Food Additives - metabolism
Food and Nutrition
Intestine, Small - metabolism
Life Sciences
Lipid Metabolism
Liver - metabolism
Male
Mice
Mice, Inbred C57BL
Milk - chemistry
nutrition
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Summary:Additives stabilize or improve the organoleptic or functional properties (or both) of many dairy products including whipping cream. Their influence on the metabolic effect of dairy cream is scarcely known. We tested the hypothesis that added emulsifier (lactic acid esters of mono- and diglycerides; MAG/DAG), thickener (carrageenan, CGN), or both, could modify the metabolic effect, notably in the intestine and liver. Nine-week-old male C57Bl/6J mice were fed UHT cream (indirect treatment) mixed with nonlipidic powder (final: 13% milkfat) for 1 or 4 wk. We compared creams (1) without additive (Ctl), (2) with thickener (Th), 0.02% of κ-CGN, and (3) with both thickener and emulsifier, 0.1% of MAG/DAG esters (Th/Em). We analyzed plasma parameters, intestine, and liver. Fasting glycemia, insulinemia, triglyceridemia, nonesterified fatty acids, body weight gain, and liver weight did not differ among groups. After 1 wk, Th/Em had higher expression in the duodenum of some of the genes involved in (1) intestinal lipid absorption and (2) tight junction proteins versus Ctl and Th. After 4 wk, mucus cell number in the small intestine was higher in Th/Em versus Ctl and Th. Genes involved in endoplasmic reticulum (ER) stress in the duodenum were more expressed in Th/Em after 1 wk. After 4 wk, in the colon, a higher expression of ER stress genes was observed for Th versus Th/Em and Ctl. Liver damage score was not altered by additives. Adding both CGN (0.02%) and MAG/DAG esters (0.1%) in dairy cream did not result in deleterious outcomes in mice after 4 wk regarding lipid metabolism, intestinal permeability, and liver disorders. The longer term effect of intestinal ER stress modulation deserves further investigation.
ISSN:0022-0302
1525-3198
DOI:10.3168/jds.2018-14783