Using temperature and time criteria to control the effectiveness of continuous thermal sanitation of piggery effluent in terms of set microbial indicators

Aim: To determine the minimal conditions (temperature–time), necessary to achieve set sanitation targets for selected microbial indicators during the continuous thermal treatment of pig slurry. Methods and Results: The effectiveness of thermal treatment between 55 and 96°C was studied using Escheric...

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Bibliographic Details
Published in:Journal of applied microbiology 2011-12, Vol.111 (6), p.1492-1504
Main Authors: Cunault, C, Pourcher, A.M, Burton, C.H
Format: Article
Language:English
Subjects:
Animals
bacteria
bacteriophages
Bacteriophages - growth & development
bacteriophages, Clostridium
Biological and medical sciences
Clostridium
Clostridium botulinum
Clostridium botulinum - growth & development
Colony Count, Microbial
continuous treatment
decontamination
Enterococcaceae - growth & development
Enterococcus
Environmental Sciences
Escherichia coli
Escherichia coli - growth & development
Fundamental and applied biological sciences. Psychology
genes
heat
heat exchangers
heat tolerance
heat treatment
Hot Temperature
hygiene
indicator bacteria
livestock wastes
Manure - microbiology
Microbiology
pathogens
pig manure
public health
ribosomal RNA
risk
sanitation
Sanitation - methods
spores
Spores, Bacterial - growth & development
Swine - microbiology
temperature
Time Factors
Waste Disposal, Fluid - methods
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Summary:Aim: To determine the minimal conditions (temperature–time), necessary to achieve set sanitation targets for selected microbial indicators during the continuous thermal treatment of pig slurry. Methods and Results: The effectiveness of thermal treatment between 55 and 96°C was studied using Escherichia coli, enterococci, sulfite‐reducing Clostridia (SRC), mesophilic culturable bacteria (MCB), F+‐specific and somatic phages. Identification of SRC and MCB was performed using 16S rRNA gene analysis. Ten minutes at 70°C or 1 h at 60°C was sufficient to reduce the vegetative bacteria by 4–5 log10, but it had little effect on somatic phages nor on spore formers, dominated by Clostridium sp. At 96°C, somatic phages were still detected, but there was a reduction of 3·1 log10 for SRC and of 1·4 log10 for MCB. At 96°C, Clostridium botulinum was identified among the thermotolerant MCB. Conclusion: Only those hygienic risks relating to mesophilic vegetative bacteria can be totally eliminated from pig slurry treated at 60°C (60 min) or 70°C (
ISSN:1364-5072
1365-2672
DOI:10.1111/j.1365-2672.2011.05144.x