Correlation between invariant chain expression level and capability to present antigen to MHC class II-restricted T cells

In this study we Investigated the role of the Invariant chain (II) in the presentation of hen egg lysozyme (HEL) and measles virus hemagglutlnln (HA) antigens to MHC class ll-restrlcted T hybridoma cells. Fibroblastic cells transfected with Ed or Ak genes, and supertransfected or not with the II gen...

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Bibliographic Details
Published in:International immunology 1991-05, Vol.3 (5), p.435-443
Main Authors: Bertolino, Patrick, Forquet, Frédérique, Pont, Suzanne, Koch, Norbert, Gerlier, Denis, Rabourdin-Combe, Chantal
Format: Article
Language:English
Subjects:
Adaptive immunology
Animals
Antigen-Presenting Cells - immunology
Antigens - metabolism
antisense oligdeoxynucleotide
Base Sequence
Chickens
Contact Inhibition
DNA, Antisense - genetics
Fibroblasts - immunology
Gene Expression Regulation
Genes, MHC Class II
Hemagglutinins, Viral - immunology
hen egg
Histocompatibility Antigens Class II - biosynthesis
Histocompatibility Antigens Class II - genetics
Histocompatibility Antigens Class II - immunology
IL-2 secretion
Immunology
Life Sciences
lysozyme
Measles virus
measles virus haemagglutinin
Molecular Sequence Data
Muramidase - immunology
Protein Processing, Post-Translational
T-cell proliferation
T-Lymphocytes - immunology
Transfection
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Summary:In this study we Investigated the role of the Invariant chain (II) in the presentation of hen egg lysozyme (HEL) and measles virus hemagglutlnln (HA) antigens to MHC class ll-restrlcted T hybridoma cells. Fibroblastic cells transfected with Ed or Ak genes, and supertransfected or not with the II gene, were used as antigen-presenting cells (APC). For every APC pair analysed, the amount of exogenous antigen needed to obtain a T-cell response was inversely correlated with the level of II expression. Exogenously provided HEL was efficiently presented by II-supertransfected APC at doses of 10 μg/ml or below. In contrast, non-ll transfected fibroblastic cells, which express a low level of endogenous II, required at least 10 times more HEL to stimulate most of the T hybridoma cells. Analogous results were also obtained using exogenous HA. Finally, two different experiments suggest that basal II expressed in fibroblastic cells Is involved in the presentation of exogenous antigen. In the first one, we showed that ll/claas II ratio was increased In high-density grown fibroblastic cells and that this increase correlates with the ability of the cells to present exogenous antigen. In the second, treating high-density grown cells with an antisense II oligodeoxynucleotide could Impair their ability to present exogenous HEL. We also examined the presentation of endogenously-synthesized HEL or HA after introduction of the antigen Into the blosynthetic pathway of the APC by transfection of HEL and HA cDNAs. There was no apparent difference In the capability of high density grown fibroblastic cells, transfected or not with II gene, to present endogenous HEL or HA. However, when decreasing the li/class II ratio by growing non-li supertransfected cells at low density, we obtained evidence for a presentation pathway of endogenous HEL dependent on II expression.
ISSN:0953-8178
1460-2377
DOI:10.1093/intimm/3.5.435