Dendritic Cells Enhance the Differentiation of Naïve B Cells into Plasma Cells In Vitro

We have shown previously that in vitro‐generated human dendritic cells have an effect on the response of B cells at various stages of their differentiation. In a culture system described for the in vitro induction of plasma‐cell differentiation, it was reported that naïve B cells have a poor propens...

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Bibliographic Details
Published in:Scandinavian journal of immunology 1998-12, Vol.48 (6), p.563-570
Main Authors: Fayette, J, Durand, I, Bridon, J M, Arpin, C, Dubois, B, Caux, C, Liu, Y J, Banchereau, J, Brière, F
Format: Article
Language:English
Subjects:
Adaptive immunology
B-Lymphocytes - cytology
B-Lymphocytes - drug effects
B-Lymphocytes - metabolism
CD40 Antigens - metabolism
Cell Differentiation
Dendritic Cells - metabolism
Humans
Immunoglobulin D - metabolism
Immunoglobulins - biosynthesis
Immunology
Immunotherapy
Innate immunity
Interleukin-10 - metabolism
Interleukin-10 - pharmacology
Interleukin-12 - metabolism
Interleukin-2 - pharmacology
Interleukin-6 - metabolism
Life Sciences
Lymphocyte Activation
Plasma Cells - cytology
Quantitative Methods
Vaccinology
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Summary:We have shown previously that in vitro‐generated human dendritic cells have an effect on the response of B cells at various stages of their differentiation. In a culture system described for the in vitro induction of plasma‐cell differentiation, it was reported that naïve B cells have a poor propensity to differentiate into plasma cells. In such a culture system, 12% of naïve B cells differentiated into plasma cells in the presence of IL‐2 and IL‐10, despite the interruption of CD40 signalling which is necessary for plasma‐cell differentiation. However, as reported herein, naïve B cells differentiated fully into plasma cells in response to dendritic cells. Addition of dendritic cells enhanced this differentiation strikingly by recruiting 57% of B cells as plasma cells producing IgM, but also IgG and IgA. In this model, dendritic cells act in synergy with IL‐2 at an early stage of CD40‐dependent B‐cell differentiation, while IL‐2 and IL‐10 act together, at a later stage, in the generation of plasma cells in a CD40‐independent manner. Thus, in addition to the key role played by dendritic cells in the initiation of T‐cell responses, our results suggest that dendritic cells regulate humoral responses.
ISSN:0300-9475
1365-3083
DOI:10.1046/j.1365-3083.1998.00471.x