Mechanical stress rapidly induces E-resveratrol and E-piceatannol biosynthesis in grape canes stored as a freshly-pruned byproduct

•A method to induce stilbenoids in freshly-pruned grape canes is described.•A mechanical stress is applied by cutting the canes in short length segments.•E-resveratrol and E-piceatannol rapidly accumulate.•A global defense response is observed.•The optimal storage period is shortened from 6 to 2week...

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Bibliographic Details
Published in:Food chemistry 2018-02, Vol.240, p.1022-1027
Main Authors: Billet, Kévin, Houillé, Benjamin, Besseau, Sébastien, Mélin, Céline, Oudin, Audrey, Papon, Nicolas, Courdavault, Vincent, Clastre, Marc, Giglioli-Guivarc'h, Nathalie, Lanoue, Arnaud
Format: Article
Language:English
Subjects:
Biotechnology
Byproduct
Chemical and Process Engineering
E-piceatannol
E-resveratrol
Engineering Sciences
Grape canes
Life Sciences
Mechanical wounding
Plant breeding
Stilbenes
Stilbenoid biosynthesis
Stress induction
Stress, Mechanical
Vegetal Biology
Vitis
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Summary:•A method to induce stilbenoids in freshly-pruned grape canes is described.•A mechanical stress is applied by cutting the canes in short length segments.•E-resveratrol and E-piceatannol rapidly accumulate.•A global defense response is observed.•The optimal storage period is shortened from 6 to 2weeks. Grape canes represent a promising source of bioactive phytochemicals. However the stabilization of the raw material after pruning remains challenging. We recently reported the induction of stilbenoid metabolism after winter pruning including a strong accumulation of E-resveratrol and E-piceatannol during the first six weeks of storage. In the present study, the effect of mechanical wounding on freshly-pruned canes was tested to increase the induction of stilbenoid metabolism. Cutting the grape canes in short segments immediately after pruning triggered a transient expression of phenylalanine ammonia-lyase (PAL) and stilbene synthase (STS) genes, followed by a rapid accumulation of E-resveratrol and E-piceatannol. The degree of stilbenoid induction was related to the intensity of mechanical wounding. Data suggest that a global defense response is triggered involving jasmonate signaling, PR proteins and stilbenoid metabolism. Mechanical wounding of freshly-pruned canes drastically shortens the time required to reach maximal stilbenoid accumulation from 6 to 2weeks.
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2017.07.105