A novel method to obtain protein release from porous polymer scaffolds: emulsion coating

To obtain the controlled release of proteins from macro-porous polymeric scaffolds, a novel emulsion-coating method has been developed. In this process, a water-in-oil emulsion, from an aqueous protein solution and a polymer solution, is forced through a prefabricated scaffold by applying a vacuum....

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Bibliographic Details
Published in:Journal of controlled release 2003-02, Vol.87 (1), p.57-68
Main Authors: Sohier, J., Haan, R.E., de Groot, K., Bezemer, J.M.
Format: Article
Language:English
Subjects:
Animals
Bioengineering
Biological and medical sciences
Biomaterials
Chickens
Coating
Controlled release
Delayed-Action Preparations - chemistry
Delayed-Action Preparations - pharmacokinetics
Drug Delivery Systems - methods
Emulsions
General pharmacology
Growth factors
Life Sciences
Medical sciences
Multiblock copolymers
Muramidase - chemistry
Muramidase - pharmacokinetics
Pharmaceutical technology. Pharmaceutical industry
Pharmacology. Drug treatments
Polymers - chemistry
Polymers - pharmacokinetics
Porosity - drug effects
Proteins - chemistry
Proteins - pharmacokinetics
Scaffolds
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Summary:To obtain the controlled release of proteins from macro-porous polymeric scaffolds, a novel emulsion-coating method has been developed. In this process, a water-in-oil emulsion, from an aqueous protein solution and a polymer solution, is forced through a prefabricated scaffold by applying a vacuum. After solvent evaporation, a polymer film, containing the protein, is then deposited on the porous scaffold surface. This paper reports the effect of processing parameters on the emulsion coating characteristics, scaffold structure, and protein release and stability. Poly(ether–ester) multiblock copolymers were chosen as the polymer matrix for both scaffolds and coating. Macro-porous scaffolds, with a porosity of 77 vol% and pores of approximately 500 μm were prepared by compression moulding/salt leaching. A micro-porous, homogeneous protein-loaded coating could be obtained on the scaffold surface. Due to the coating, the scaffold porosity was decreased, whereas the pore interconnection was increased. A model protein (lysozyme) could effectively be released in a controlled fashion from the scaffolds. Complete lysozyme release could be achieved within 3 days up to more than 2 months by adjusting the coated emulsion parameters. In addition, the coating process did not reduce the enzymatic activity. This new method appears to be promising for tissue engineering applications.
ISSN:0168-3659
1873-4995
DOI:10.1016/S0168-3659(02)00350-4