Adaptation of Pseudomonas aeruginosa to a pulsed light-induced stress

Aims:  Pulsed light (PL) technology is an efficient surface decontamination process. Used in low transmitted energy conditions, PL induces a stress that can be perceived by bacteria. The effect of such a PL stress was investigated on the highly environmental adaptable germ Pseudomonas aeruginosa PAO...

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Bibliographic Details
Published in:Journal of applied microbiology 2012-03, Vol.112 (3), p.502-511
Main Authors: Massier, S., Rincé, A., Maillot, O., Feuilloley, M.G.J., Orange, N., Chevalier, S.
Format: Article
Language:English
Subjects:
adaptation
Adaptation, Physiological
Bacteriology
Biological and medical sciences
Decontamination - methods
Fundamental and applied biological sciences. Psychology
Life Sciences
Light
Microbial Viability - radiation effects
Microbiology
Microbiology and Parasitology
Mutation Rate
proteome
Proteome - analysis
Pseudomonas aeruginosa
Pseudomonas aeruginosa - genetics
Pseudomonas aeruginosa - metabolism
Pseudomonas aeruginosa - radiation effects
pulsed light technology
pulsed light-induced stress
Stress, Physiological
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Summary:Aims:  Pulsed light (PL) technology is an efficient surface decontamination process. Used in low transmitted energy conditions, PL induces a stress that can be perceived by bacteria. The effect of such a PL stress was investigated on the highly environmental adaptable germ Pseudomonas aeruginosa PAO1. Methods and Results:  Pulses of transmitted energy (fluence) reaching 1·8 J cm−2 can kill 109 bacteria. Application of a lower sublethal PL dose allowed the bacteria to resist and survive more efficiently to a subsequent dose of PL. This sublethal dose was not increasing the mutation frequency of Ps. aeruginosa, but altered the abundance of 15 proteins as revealed by a global proteome analysis, including stress‐induced proteins, phage‐related proteins, energy and carbon metabolisms, cell motility, and transcription and translation regulators. Conclusions:  A response to a low‐energy PL dose takes place in Ps. aeruginosa, reducing the energy conversion systems, while increasing transcription and translation processes to produce proteins involved in chaperone mechanisms and phage‐related proteins, probably to protect the bacterium against a new PL‐induced stress. Significance and Impact of the Study:  Taken together, these results suggest that a low‐energy PL dose is sufficient to provoke adaptation of Ps. aeruginosa, leading to enhancing its resistance to a subsequent lethal treatment.
ISSN:1364-5072
1365-2672
DOI:10.1111/j.1365-2672.2011.05224.x