Effects of a pulsed light‐induced stress on Enterococcus faecalis

Aims Pulsed light (PL) technology is a surface decontamination process that can be used on food, packaging or water. PL efficiency may be limited by its low degree of penetration or because of a shadow effect. In these cases, surviving bacteria will be able to perceive PL as a stress. Such a stress...

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Bibliographic Details
Published in:Journal of applied microbiology 2013-01, Vol.114 (1), p.186-195
Main Authors: Massier, S., Bouffartigues, E., Rincé, A., Maillot, O., Feuilloley, M.G.J., Orange, N., Chevalier, S.
Format: Article
Language:English
Subjects:
Adaptation, Physiological
Bacteria
Bacterial Proteins - genetics
Bacteriology
Biological and medical sciences
biotechnology
Decontamination - methods
Enterococcus faecalis
Enterococcus faecalis - genetics
Enterococcus faecalis - radiation effects
Food industries
food preservation
Fundamental and applied biological sciences. Psychology
General aspects
Hygiene and safety
Life Sciences
Light
microbial contamination
Microbial Viability
Microbiology
Microbiology and Parasitology
Mutation
Mutation Rate
Proteome - analysis
proteomics
Stress, Physiological
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Summary:Aims Pulsed light (PL) technology is a surface decontamination process that can be used on food, packaging or water. PL efficiency may be limited by its low degree of penetration or because of a shadow effect. In these cases, surviving bacteria will be able to perceive PL as a stress. Such a stress was mimicked using low transmitted energy conditions, and its effects were investigated on the highly environmental adaptable bacterium Enterococcus faecalis V583. Methods and Results In these laboratory conditions, a complete decontamination of the artificially inoculated medium was performed using energy doses as low as 1·8 J cm−2, while a treatment of 0·5, 1 and 1·2 J cm−2 led to a 2·2, 6 and 7‐log10 CFU ml−1 reduction in the initial bacterial population, respectively. Application of a 0·5 J cm−2 pretreatment allowed the bacteria to resist more efficiently a 1·2 J cm−2 subsequent PL dose. This 0·5 J cm−2 treatment increased the bacterial mutation frequency and affected the abundance of 19 proteins as revealed by a global proteome analysis. Conclusions Enterococcus faecalis is able to adapt to a PL treatment, providing a molecular response to low‐energy PL dose, leading to enhanced resistance to a subsequent treatment and increasing the mutation frequency. Significance and Impact of the Study This study gives further insights on Ent. faecalis capacities to adapt and to resist to stress.
ISSN:1364-5072
1365-2672
DOI:10.1111/jam.12029