Self-etching Increases Matrix Metalloproteinase Expression in the Dentin-Pulp Complex

In adhesive restorations, one major problem is hybrid layer degradation. At present, this deterioration is explained by the activation of the endogenous matrix metalloproteinases (MMPs) present in dentin due to the acidic property of adhesive systems. We hypothesized that self-etching adhesive shoul...

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Bibliographic Details
Published in:Journal of dental research 2009-01, Vol.88 (1), p.77-82
Main Authors: Lehmann, N., Debret, R., Roméas, A., Magloire, H., Degrange, M., Bleicher, F., Sommer, P., Seux, D.
Format: Article
Language:English
Subjects:
Adolescent
Blotting, Western
Coloring Agents
Composite Resins - chemistry
Composite Resins - pharmacology
Dental Pulp - drug effects
Dental Pulp - enzymology
Dental Pulp - ultrastructure
Dentin - drug effects
Dentin - enzymology
Dentin - ultrastructure
Dentin-Bonding Agents - chemistry
Dentin-Bonding Agents - pharmacology
Dentistry
Enzyme Precursors - drug effects
Gelatinases - drug effects
Humans
Immunohistochemistry
Isoenzymes - drug effects
Life Sciences
Light-Curing of Dental Adhesives
Materials Testing
Matrix Metalloproteinase 2 - drug effects
Matrix Metalloproteinase 9 - drug effects
Odontoblasts - drug effects
Odontoblasts - enzymology
Odontoblasts - ultrastructure
Surface Properties
Tissue Culture Techniques
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Summary:In adhesive restorations, one major problem is hybrid layer degradation. At present, this deterioration is explained by the activation of the endogenous matrix metalloproteinases (MMPs) present in dentin due to the acidic property of adhesive systems. We hypothesized that self-etching adhesive should also stimulate the expression of MMPs in odontoblasts. In cultured tooth slices, we evaluated the changes in MMP-2 and proMMP-9 expression in the dentin-pulp complex after self-etching adhesive treatment on dentin cavities in immunochemistry and by zymography. The treatment resulted in increased MMP-2 expression in odontoblasts, as shown by immunohistochemistry. Zymography showed increased proMMP-9 and MMP-2 in dentin under self-etching treatment when pulp was present. These results showed that self-etching adhesive stimulates the secretion of MMPs from the dentin-pulp complex and, more precisely, by odontoblasts, suggesting that odontoblasts participate in hybrid layer degradation.
ISSN:0022-0345
1544-0591
DOI:10.1177/0022034508327925