Ca 2+ /CALMODULIN‐DEPENDENT PROTEIN KINASE II IN THE COCKROACH Periplaneta americana : IDENTIFICATION OF FIVE ISOFORMS AND THEIR TISSUES DISTRIBUTION

Calcium/calmodulin‐dependent protein kinase II ( C a MKII ) is a key kinase that transduces C a 2+ signals into downstream effects acting on a range of cellular processes in nervous system and muscular tissues. In insects, different C a MKII isoforms have been reported in D rosophila melanogaster, A...

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Bibliographic Details
Published in:Archives of insect biochemistry and physiology 2013-07, Vol.83 (3), p.138-150
Main Authors: Taillebois, Emiliane, Heuland, Emilie, Bourdin, Céline M., Griveau, Audrey, Quinchard, Sophie, Tricoire‐Leignel, Helene, Legros, Christian, Thany, Steeve H.
Format: Article
Language:English
Subjects:
Alternative Splicing
Animals
Biochemistry, Molecular Biology
Calcium-Calmodulin-Dependent Protein Kinase Type 2
Cloning, Molecular
Computational Biology
DNA Primers
Environmental Sciences
Gene Expression Regulation
Genetics
Life Sciences
Periplaneta
Protein Isoforms
Reverse Transcriptase Polymerase Chain Reaction
Tissue Distribution
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Summary:Calcium/calmodulin‐dependent protein kinase II ( C a MKII ) is a key kinase that transduces C a 2+ signals into downstream effects acting on a range of cellular processes in nervous system and muscular tissues. In insects, different C a MKII isoforms have been reported in D rosophila melanogaster, A pis florae, B ombus terrestris, and B ombus impatiens but little is known on the organization and tissue‐specific expression of these isoforms with the exception of D rosophila. The present study reports the cloning of five C a MKII splice variants issued from a single gene and their tissue‐specific expression in the cockroach P eriplaneta americana. Each C a MKII isoform shared 82–90% identity with D rosophila C a MKII isoforms and accordingly were named P a C a MKII ‐ A , P a C a MKII ‐ B , P a C a MKII ‐ C , P a C a MKII ‐ D , and P a C a MKII ‐ E . P a C a MKII ‐ A and P a C a MKII ‐ D isoforms are ubiquitously expressed in all tissues, but some such as P a C a MKII ‐ B and P a C a MKII ‐ C are preferentially expressed in the nerve cord and muscle. In addition, using single‐cell reverse transcriptase‐polymerase chain reaction ( RT ‐ PCR ), we found a tissue‐specific expression of P a C a MKII ‐ E in the dorsal unpaired median neurons. Alternative splicing of P a C a MKII transcripts is likely a common mechanism in insects to control the pattern of isoform expression in the different tissues.
ISSN:0739-4462
1520-6327
DOI:10.1002/arch.21102