Identification of protective B-cell epitopes of Atroxlysin-I: A metalloproteinase from Bothrops atrox snake venom

Abstract Atroxlysin-I (Atr-I) is a hemorrhagic snake venom metalloproteinase (SVMP) from Bothrops atrox venom, the snake responsible for the majority of bites in the north region of South America. SVMPs like Atr-I produce toxic effects in victims including hemorrhage, inflammation, necrosis and bloo...

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Bibliographic Details
Published in:Vaccine 2016-03, Vol.34 (14), p.1680-1687
Main Authors: Schneider, F.S, de Almeida Lima, S, Reis de Ávila, G, Castro, K.L, Guerra-Duarte, C, Sanchez, E.F, Nguyen, C, Granier, C, Molina, F, Chávez-Olortegui, C
Format: Article
Language:English
Subjects:
Allergy and Immunology
Amino Acid Sequence
Animals
Antibodies, Neutralizing - immunology
Antivenins - immunology
Bothrops
Cellulose
Cross Reactions
Epitope Mapping
Epitopes, B-Lymphocyte - immunology
Ethanol
Hemorrhage
Immunization
Immunoglobulins
Life Sciences
Linear B-cell epitope mapping
Metalloendopeptidases - immunology
Mice, Inbred BALB C
Molecular Sequence Data
Molecular weight
Neutralization Tests
Peptide synthesis
Peptides
Peptides - immunology
Protein Structure, Tertiary
Proteins
Snake venom metalloproteinases
Snake Venoms - immunology
Venom
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Summary:Abstract Atroxlysin-I (Atr-I) is a hemorrhagic snake venom metalloproteinase (SVMP) from Bothrops atrox venom, the snake responsible for the majority of bites in the north region of South America. SVMPs like Atr-I produce toxic effects in victims including hemorrhage, inflammation, necrosis and blood coagulation deficiency. Mapping of B-cell epitopes in SVMPs might result in the identification of non-toxic molecules capable of inducing neutralizing antibodies and improving the anti-venom therapy. Here, using the SPOT-synthesis technique we identified two epitopes located in the N-ter region of Atr-I (AtrEp1—22 YNGNSDKIRRRIHQM36 ; and AtrEp2—55 GVEIWSNKDLINVQ68 ). Based on the sequence of AtrEp1 and AtrEp2 a third peptide named Atr-I biepitope (AtrBiEp) was designed and synthesized (23 NGNSDKIRRRIH34 GG55 GVEIWSNKDLINVQ68 ). AtrBiEp was used to immunize BALB/c mice. Anti-AtrBiEp serum cross-reacted against Atr-I in western blot and was able to fully neutralize the hemorrhagic activity of Atr-I. Our results provide a rational basis for the identification of neutralizing epitopes on Atr-I snake venom toxin and show that the use of synthetic peptides could improve the generation of immuno-therapeutics.
ISSN:0264-410X
1873-2518
0264-410X
DOI:10.1016/j.vaccine.2016.02.035