Partial in vitro analysis of toxic and antigenic activities of eleven Peruvian pitviper snake venoms

This work used eleven Peruvian snake venoms (Bothrops andianus, Bothrops atrox, Bothrops barnetti, Bothrops castelnaudi, Bothriopsis chloromelas, Bothrocophias microphthalmus, Bothrops neuwiedi, Bothriopsis oligolepis, Bothriopsis peruviana, Bothrops pictus and Bothriopsis taeniata) to perform in vi...

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Bibliographic Details
Published in:Toxicon (Oxford) 2015-12, Vol.108, p.84-96
Main Authors: Guerra-Duarte, C., Lopes-Peixoto, J., Fonseca-de-Souza, B.R., Stransky, S., Oliveira, D., Schneider, F.S., Lopes-de-Souza, L., Bonilla, C., Silva, W., Tintaya, B., Yarleque, A., Chávez-Olórtegui, C.
Format: Article
Language:English
Subjects:
Animals
Antivenins - pharmacology
Assaying
Blotting, Western
Bothrops
Bothrops neuwiedi
Brazil
Cell Line
Cercopithecus aethiops
Crotalid Venoms - enzymology
Crotalid Venoms - immunology
Crotalid Venoms - toxicity
Enzyme-Linked Immunosorbent Assay
HeLa Cells
Humans
Hyaluronoglucosaminidase - metabolism
In vitro testing
In vitro assays
L-Amino Acid Oxidase - metabolism
Life Sciences
Metalloproteases - metabolism
Microphthalmus
Oligolepis
Oxidase
Peru
Peruvian snakes
Phospholipase
Phospholipases A2 - metabolism
Serine Proteases - metabolism
Snakes
Toxicity
Venom characterization
Vero Cells
Viability
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Summary:This work used eleven Peruvian snake venoms (Bothrops andianus, Bothrops atrox, Bothrops barnetti, Bothrops castelnaudi, Bothriopsis chloromelas, Bothrocophias microphthalmus, Bothrops neuwiedi, Bothriopsis oligolepis, Bothriopsis peruviana, Bothrops pictus and Bothriopsis taeniata) to perform in vitro experimentation and determine its main characteristics. Hyaluronidase (HYAL), phospholipase A2 (PLA2), snake venom metalloproteinase (SVMP), snake venom serine protease (SVSP) and l-amino acid oxidase (LAAO) activities; toxicity by cell viability assays using MGSO3, VERO and HeLa cell lineages; and crossed immunoreactivity with Peruvian (PAV) and Brazilian (BAV) antibothropic polyvalent antivenoms, through ELISA and Western Blotting assays, were determined. Results show that the activities tested in this study were not similar amongst the venoms and each species present their own peculiarities, highlighting the diversity within Bothrops complex. All venoms were capable of reducing cell viability of all tested lineages. It was also demonstrated the crossed recognition of all tested venoms by both antivenoms. •Enzymatic activities from eleven Peruvian snakes were characterized in vitro.•The eleven venoms showed very different enzymatic profiles.•Commercial bothropic antivenoms from Peru and Brazil reacted with the eleven venoms.•All tested venoms were highly cytotoxic against cancer and normal cell lineages.•Cytotoxicity was highly correlated with l-amino acid oxidase activity.
ISSN:0041-0101
1879-3150
0041-0101
DOI:10.1016/j.toxicon.2015.09.007