NG2 and Olig2 Expression Provides Evidence for Phenotypic Deregulation of Cultured Central Nervous System and Peripheral Nervous System Neural Precursor Cells

Neural stem cells cultured with fibroblast growth factor 2 (FGF2)/epidermal growth factor (EGF) generate clonal expansions called neurospheres (NS), which are widely used for therapy in animal models. However, their cellular composition is still poorly defined. Here, we report that NS derived from s...

Full description

Saved in:
Bibliographic Details
Published in:Stem cells (Dayton, Ohio) Ohio), 2007-02, Vol.25 (2), p.340-353
Main Authors: Dromard, Cecile, Bartolami, Sylvain, Deleyrolle, Loïc, Takebayashi, Hirohide, Ripoll, Chantal, Simonneau, Lionel, Prome, Sylvie, Puech, Sylvie, Tran Van Ba, Christophe, Duperray, Christophe, Valmier, Jean, Privat, Alain, Hugnot, Jean‐Philippe
Format: Article
Language:English
Subjects:
Animals
Antigens - metabolism
Basic Helix-Loop-Helix Transcription Factors - genetics
Basic Helix-Loop-Helix Transcription Factors - metabolism
Cell Differentiation
Cells, Cultured
Central Nervous System - cytology
Cytometry Plasticity
Embryo, Mammalian - cytology
Gangliosides - metabolism
Gene Expression Regulation
High Mobility Group Proteins - metabolism
Life Sciences
Mice
Models, Biological
Nerve Tissue Proteins - genetics
Nerve Tissue Proteins - metabolism
Neural stem cells
Neuroglia - cytology
Neuroglia - metabolism
Neurons - cytology
Neurons - metabolism
Neurons and Cognition
Neurospheres
NG2
Oligodendrocyte Transcription Factor 2
Oligodendrocytes
Peripheral nervous system
Peripheral Nervous System - cytology
Phenotype
Proteoglycan
Proteoglycans - metabolism
Receptor, Platelet-Derived Growth Factor alpha - genetics
Receptor, Platelet-Derived Growth Factor alpha - metabolism
RNA, Messenger - genetics
RNA, Messenger - metabolism
SOX9 Transcription Factor
Spinal Cord - cytology
Spinal Cord - embryology
Stem Cells - cytology
Stem Cells - metabolism
Transcription Factors - metabolism
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Neural stem cells cultured with fibroblast growth factor 2 (FGF2)/epidermal growth factor (EGF) generate clonal expansions called neurospheres (NS), which are widely used for therapy in animal models. However, their cellular composition is still poorly defined. Here, we report that NS derived from several embryonic and adult central nervous system (CNS) regions are composed mainly of remarkable cells coexpressing radial glia markers (BLBP, RC2, GLAST), oligodendrogenic/neurogenic factors (Mash1, Olig2, Nkx2.2), and markers that in vivo are typical of the oligodendrocyte lineage (NG2, A2B5, PDGFR‐α). On NS differentiation, the latter remain mostly expressed in neurons, together with Olig2 and Mash1. Using cytometry, we show that in growing NS the small population of multipotential self‐renewing NS‐forming cells are A2B5+ and NG2+. Additionally, we demonstrate that these NS‐forming cells in the embryonic spinal cord were initially NG2− and rapidly acquired NG2 in vitro. NG2 and Olig2 were found to be rapidly induced by cell culture conditions in spinal cord neural precursor cells. Olig2 expression was also induced in astrocytes and embryonic peripheral nervous system (PNS) cells in culture after EGF/FGF treatment. These data provide new evidence for profound phenotypic modifications in CNS and PNS neural precursor cells induced by culture conditions.
ISSN:1066-5099
1549-4918
DOI:10.1634/stemcells.2005-0556