Genetic diversity among dairy lactococcal strains investigated by polymerase chain reaction with three arbitrary primers

Randomly amplified polymorphic DNA (RAPD) has been used for the rapid typing of Lactococcus lactis strains isolated from raw milk from the Camembert region of Normandy. It is thought that the diversity and perhaps the area strain specificity due to climatic and geographical factors of such wild‐type...

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Bibliographic Details
Published in:Journal of applied microbiology 1999-03, Vol.86 (3), p.514-520
Main Authors: Mangin, I., Corroler, D., Reinhardt, A., Gueguen, M.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
DNA Primers
DNA, Bacterial - analysis
Food industries
Fundamental and applied biological sciences. Psychology
Genetic Testing - methods
Genetic Variation
Lactococcus lactis - genetics
Lactococcus lactis cremoris
Lactococcus lactis lactis
Life Sciences
Milk - microbiology
Milk and cheese industries. Ice creams
Polymerase Chain Reaction - methods
Polymorphism, Genetic
Species Specificity
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Summary:Randomly amplified polymorphic DNA (RAPD) has been used for the rapid typing of Lactococcus lactis strains isolated from raw milk from the Camembert region of Normandy. It is thought that the diversity and perhaps the area strain specificity due to climatic and geographical factors of such wild‐type lactococcal strains could contribute to the flavour differences and specific features detected for the same product in different areas. The patterns from 58 isolates were analysed by UPGMA dendrograms. At a similarity level of 50%, four RAPD clusters were distinguished. Clusters 1 and 2 contained strains of subspecies lactis and cluster 3 contained strains related to the C2 strain which is genetically cremoris but phenotypically lactis. The type strain of cremoris subspecies was significantly differentiated from these strains with primers P2 and P3. Thus, there was a real genetic diversity in pattern, making it possible to detect potential typical RAPD fragments.
ISSN:1364-5072
1365-2672
DOI:10.1046/j.1365-2672.1999.00699.x