Induction of sulfadiazine resistance in vitro in Toxoplasma gondii

[Display omitted] ► Adaptation of a specific Toxoplasma enzyme immunoassay on Vero cells. ► Determination of IC50 values on several T. gondii strains. ► Confirmation of sulfadiazine susceptibilities on T. gondii strains. ► Development in vitro of two sulfadiazine resistant strains: RH-RSDZ and ME-49...

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Bibliographic Details
Published in:Experimental parasitology 2013-02, Vol.133 (2), p.131-136
Main Authors: Doliwa, C., Escotte-Binet, S., Aubert, D., Velard, F., Schmid, A., Geers, R., Villena, I.
Format: Article
Language:English
Subjects:
Animals
Antiprotozoal Agents - pharmacology
Cercopithecus aethiops
Drug Resistance
drugs
Enzyme-Linked Immunosorbent Assay
In vitro
induced resistance
Inhibitory Concentration 50
Life Sciences
Parasitic Sensitivity Tests
parasitology
Pressure
resistance mechanisms
Sulfadiazine
Sulfadiazine - pharmacology
Toxoplasma - drug effects
Toxoplasma gondii
Vero Cells
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Summary:[Display omitted] ► Adaptation of a specific Toxoplasma enzyme immunoassay on Vero cells. ► Determination of IC50 values on several T. gondii strains. ► Confirmation of sulfadiazine susceptibilities on T. gondii strains. ► Development in vitro of two sulfadiazine resistant strains: RH-RSDZ and ME-49-RSDZ. We induced sulfadiazine resistance in two sulfadiazine sensitive strains of Toxoplasma gondii, RH (Type I) and ME-49 (Type II) in vitro by using drug pressure. At first, sulfadiazine susceptibility of the two sensitive strains and two naturally resistant strains of T. gondii was evaluated on Vero cells using an enzyme-linked immunosorbent assay (ELISA). The IC50 values of sulfadiazine were 77μg/mL for RH, 51μg/mL for ME-49 and higher than 1000μg/mL for the two natural resistant strains. Secondly, induced resistance of the strains by gradually increase sulfadiazine concentration was verified by this test, which resulted IC50 values at higher than 1000μg/mL. In conclusion we developed in vitro two sulfadiazine resistant strains called RH-RSDZ and ME-49-RSDZ. These strains resistant to sulfadiazine would be useful to characterize resistance mechanisms to sulfadiazine.
ISSN:0014-4894
1090-2449
DOI:10.1016/j.exppara.2012.11.019