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Variation in traction forces during cell cycle progression
Background Information Tissue morphogenesis results from the interplay between cell growth and mechanical forces. While the impact of geometrical confinement and mechanical forces on cell proliferation has been fairly well characterised, the inverse relationship is much less understood. Here, we inv...
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| Published in: | Biology of the cell 2018-04, Vol.110 (4), p.91-96 |
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| Main Authors: | , , , , , , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c4806-21f2d21e66c84d8f0187d37b1f3441ed39e116f42795f6ed9f49b960bcb92b1f3 |
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| cites | cdi_FETCH-LOGICAL-c4806-21f2d21e66c84d8f0187d37b1f3441ed39e116f42795f6ed9f49b960bcb92b1f3 |
| container_end_page | 96 |
| container_issue | 4 |
| container_start_page | 91 |
| container_title | Biology of the cell |
| container_volume | 110 |
| creator | Vianay, Benoit Senger, Fabrice Alamos, Simon Anjur‐Dietrich, Maya Bearce, Elizabeth Cheeseman, Bevan Lee, Lisa Théry, Manuel |
| description | Background Information
Tissue morphogenesis results from the interplay between cell growth and mechanical forces. While the impact of geometrical confinement and mechanical forces on cell proliferation has been fairly well characterised, the inverse relationship is much less understood. Here, we investigated how traction forces vary during cell cycle progression.
Results
Cell shape was constrained on micropatterned substrates in order to distinguish variations in cell contractility from cell size increase. We performed traction force measurements of asynchronously dividing cells expressing a cell‐cycle reporter, to obtain measurements of contractile forces generated during cell division. We found that forces tend to increase as cells progress through G1, before reaching a plateau in S phase, and then decline during G2.
Conclusions
While cell size increases regularly during cell cycle progression, traction forces follow a biphasic behaviour based on specific and opposite regulation of cell contractility during early and late growth phases.
Significance
These results highlight the key role of cellular signalling in the regulation of cell contractility, independently of cell size and shape. Non‐monotonous variations of cell contractility during cell cycle progression are likely to impact the mechanical regulation of tissue homoeostasis in a complex and non‐linear manner.
Short Communication
This manuscript compares cell traction forces according to their position in their cell cycle. It shows that forces increase from early G1 to S phase. It then reveals an unexpected decrease of traction forces from S to G2. |
| doi_str_mv | 10.1111/boc.201800006 |
| format | article |
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Tissue morphogenesis results from the interplay between cell growth and mechanical forces. While the impact of geometrical confinement and mechanical forces on cell proliferation has been fairly well characterised, the inverse relationship is much less understood. Here, we investigated how traction forces vary during cell cycle progression.
Results
Cell shape was constrained on micropatterned substrates in order to distinguish variations in cell contractility from cell size increase. We performed traction force measurements of asynchronously dividing cells expressing a cell‐cycle reporter, to obtain measurements of contractile forces generated during cell division. We found that forces tend to increase as cells progress through G1, before reaching a plateau in S phase, and then decline during G2.
Conclusions
While cell size increases regularly during cell cycle progression, traction forces follow a biphasic behaviour based on specific and opposite regulation of cell contractility during early and late growth phases.
Significance
These results highlight the key role of cellular signalling in the regulation of cell contractility, independently of cell size and shape. Non‐monotonous variations of cell contractility during cell cycle progression are likely to impact the mechanical regulation of tissue homoeostasis in a complex and non‐linear manner.
Short Communication
This manuscript compares cell traction forces according to their position in their cell cycle. It shows that forces increase from early G1 to S phase. It then reveals an unexpected decrease of traction forces from S to G2.</description><identifier>ISSN: 0248-4900</identifier><identifier>EISSN: 1768-322X</identifier><identifier>DOI: 10.1111/boc.201800006</identifier><identifier>PMID: 29388708</identifier><language>eng</language><publisher>England: Wiley</publisher><subject>Cell cycle ; Cell mechanics ; Cellular Biology ; Life Sciences ; Traction forces</subject><ispartof>Biology of the cell, 2018-04, Vol.110 (4), p.91-96</ispartof><rights>2018 Société Française des Microscopies and Société de Biologie Cellulaire de France. Published by John Wiley & Sons Ltd</rights><rights>2018 Société Française des Microscopies and Société de Biologie Cellulaire de France. Published by John Wiley & Sons Ltd.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4806-21f2d21e66c84d8f0187d37b1f3441ed39e116f42795f6ed9f49b960bcb92b1f3</citedby><cites>FETCH-LOGICAL-c4806-21f2d21e66c84d8f0187d37b1f3441ed39e116f42795f6ed9f49b960bcb92b1f3</cites><orcidid>0000-0002-9968-1779 ; 0000-0003-1926-237X</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fboc.201800006$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fboc.201800006$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,315,786,790,891,27957,27958,50923,51032</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/29388708$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.science/hal-01978711$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Vianay, Benoit</creatorcontrib><creatorcontrib>Senger, Fabrice</creatorcontrib><creatorcontrib>Alamos, Simon</creatorcontrib><creatorcontrib>Anjur‐Dietrich, Maya</creatorcontrib><creatorcontrib>Bearce, Elizabeth</creatorcontrib><creatorcontrib>Cheeseman, Bevan</creatorcontrib><creatorcontrib>Lee, Lisa</creatorcontrib><creatorcontrib>Théry, Manuel</creatorcontrib><title>Variation in traction forces during cell cycle progression</title><title>Biology of the cell</title><addtitle>Biol Cell</addtitle><description>Background Information
Tissue morphogenesis results from the interplay between cell growth and mechanical forces. While the impact of geometrical confinement and mechanical forces on cell proliferation has been fairly well characterised, the inverse relationship is much less understood. Here, we investigated how traction forces vary during cell cycle progression.
Results
Cell shape was constrained on micropatterned substrates in order to distinguish variations in cell contractility from cell size increase. We performed traction force measurements of asynchronously dividing cells expressing a cell‐cycle reporter, to obtain measurements of contractile forces generated during cell division. We found that forces tend to increase as cells progress through G1, before reaching a plateau in S phase, and then decline during G2.
Conclusions
While cell size increases regularly during cell cycle progression, traction forces follow a biphasic behaviour based on specific and opposite regulation of cell contractility during early and late growth phases.
Significance
These results highlight the key role of cellular signalling in the regulation of cell contractility, independently of cell size and shape. Non‐monotonous variations of cell contractility during cell cycle progression are likely to impact the mechanical regulation of tissue homoeostasis in a complex and non‐linear manner.
Short Communication
This manuscript compares cell traction forces according to their position in their cell cycle. It shows that forces increase from early G1 to S phase. It then reveals an unexpected decrease of traction forces from S to G2.</description><subject>Cell cycle</subject><subject>Cell mechanics</subject><subject>Cellular Biology</subject><subject>Life Sciences</subject><subject>Traction forces</subject><issn>0248-4900</issn><issn>1768-322X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2018</creationdate><recordtype>article</recordtype><recordid>eNp90D1PwzAQBmALgWgpjKwoIwwpPsf1B1upgCJV6gKIzUocuxilTbEbUP89Dill4xZbp0evTi9C54CHEOe6qPWQYBA4DjtAfeBMpBkhr4eojwkVKZUY99BJCO9RUClGx6hHZCYEx6KPbl5y7_KNq1eJWyUbn-ufv629NiEpG-9Wi0Sbqkr0VlcmWft64U0IEZ2iI5tXwZzt3gF6vr97mkzT2fzhcTKepZoKzFIClpQEDGNa0FLYeCsvM16AzSgFU2bSADBLCZcjy0wpLZWFZLjQhSStGqCrLvctr9Tau2Xut6rOnZqOZ6rdYZBccIBPiPays_HOj8aEjVq60J6fr0zdBAVSZplgsYhI045qX4fgjd1nA1ZttSpWq_bVRn-xi26KpSn3-rfLCHgHvlxltv-nqdv55C_6G_iXgqk</recordid><startdate>201804</startdate><enddate>201804</enddate><creator>Vianay, Benoit</creator><creator>Senger, Fabrice</creator><creator>Alamos, Simon</creator><creator>Anjur‐Dietrich, Maya</creator><creator>Bearce, Elizabeth</creator><creator>Cheeseman, Bevan</creator><creator>Lee, Lisa</creator><creator>Théry, Manuel</creator><general>Wiley</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>1XC</scope><orcidid>https://orcid.org/0000-0002-9968-1779</orcidid><orcidid>https://orcid.org/0000-0003-1926-237X</orcidid></search><sort><creationdate>201804</creationdate><title>Variation in traction forces during cell cycle progression</title><author>Vianay, Benoit ; Senger, Fabrice ; Alamos, Simon ; Anjur‐Dietrich, Maya ; Bearce, Elizabeth ; Cheeseman, Bevan ; Lee, Lisa ; Théry, Manuel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4806-21f2d21e66c84d8f0187d37b1f3441ed39e116f42795f6ed9f49b960bcb92b1f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Cell cycle</topic><topic>Cell mechanics</topic><topic>Cellular Biology</topic><topic>Life Sciences</topic><topic>Traction forces</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vianay, Benoit</creatorcontrib><creatorcontrib>Senger, Fabrice</creatorcontrib><creatorcontrib>Alamos, Simon</creatorcontrib><creatorcontrib>Anjur‐Dietrich, Maya</creatorcontrib><creatorcontrib>Bearce, Elizabeth</creatorcontrib><creatorcontrib>Cheeseman, Bevan</creatorcontrib><creatorcontrib>Lee, Lisa</creatorcontrib><creatorcontrib>Théry, Manuel</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Hyper Article en Ligne (HAL)</collection><jtitle>Biology of the cell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vianay, Benoit</au><au>Senger, Fabrice</au><au>Alamos, Simon</au><au>Anjur‐Dietrich, Maya</au><au>Bearce, Elizabeth</au><au>Cheeseman, Bevan</au><au>Lee, Lisa</au><au>Théry, Manuel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Variation in traction forces during cell cycle progression</atitle><jtitle>Biology of the cell</jtitle><addtitle>Biol Cell</addtitle><date>2018-04</date><risdate>2018</risdate><volume>110</volume><issue>4</issue><spage>91</spage><epage>96</epage><pages>91-96</pages><issn>0248-4900</issn><eissn>1768-322X</eissn><notes>ObjectType-Article-1</notes><notes>SourceType-Scholarly Journals-1</notes><notes>ObjectType-Feature-2</notes><notes>content type line 23</notes><abstract>Background Information
Tissue morphogenesis results from the interplay between cell growth and mechanical forces. While the impact of geometrical confinement and mechanical forces on cell proliferation has been fairly well characterised, the inverse relationship is much less understood. Here, we investigated how traction forces vary during cell cycle progression.
Results
Cell shape was constrained on micropatterned substrates in order to distinguish variations in cell contractility from cell size increase. We performed traction force measurements of asynchronously dividing cells expressing a cell‐cycle reporter, to obtain measurements of contractile forces generated during cell division. We found that forces tend to increase as cells progress through G1, before reaching a plateau in S phase, and then decline during G2.
Conclusions
While cell size increases regularly during cell cycle progression, traction forces follow a biphasic behaviour based on specific and opposite regulation of cell contractility during early and late growth phases.
Significance
These results highlight the key role of cellular signalling in the regulation of cell contractility, independently of cell size and shape. Non‐monotonous variations of cell contractility during cell cycle progression are likely to impact the mechanical regulation of tissue homoeostasis in a complex and non‐linear manner.
Short Communication
This manuscript compares cell traction forces according to their position in their cell cycle. It shows that forces increase from early G1 to S phase. It then reveals an unexpected decrease of traction forces from S to G2.</abstract><cop>England</cop><pub>Wiley</pub><pmid>29388708</pmid><doi>10.1111/boc.201800006</doi><tpages>6</tpages><orcidid>https://orcid.org/0000-0002-9968-1779</orcidid><orcidid>https://orcid.org/0000-0003-1926-237X</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Biology of the cell, 2018-04, Vol.110 (4), p.91-96 |
| issn | 0248-4900 1768-322X |
| language | eng |
| recordid | cdi_hal_primary_oai_HAL_hal_01978711v1 |
| source | Wiley-Blackwell Journals |
| subjects | Cell cycle Cell mechanics Cellular Biology Life Sciences Traction forces |
| title | Variation in traction forces during cell cycle progression |
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