Transcriptional Control of the Human Pregnancy-specific Glycoprotein 5 Gene is Dependent on Two GT-boxes Recognized by the Ubiquitous Specificity Protein 1 (Sp1) Transcription Factor

Pregnancy-specific glycoprotein 5 gene (PSG-5) belongs to the human pregnancy-specific glycoprotein family, encoded by eleven highly similar and transcriptionally active genes. High levels of PSG biosynthesis are restricted to the placenta syncytiotrophoblast and are essential for the maintenance of...

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Bibliographic Details
Published in:Placenta (Eastbourne) 2004, Vol.25 (1), p.9-19
Main Authors: Nores, R., Blanchon, L., López-Dı́az, F., Bocco, J.L., Patrito, L.C., Sapin, V., Panzetta-Dutari, G.M.
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biological and medical sciences
Cercopithecus aethiops
Drosophila - genetics
Electrophoretic Mobility Shift Assay
Female
Fundamental and applied biological sciences. Psychology
Gene control
Gene Expression Regulation - physiology
Glycoproteins - genetics
Humans
Life Sciences
Molecular Sequence Data
Mother. Fetoplacental unit. Mammary gland. Milk
Placenta
Placenta - physiology
Pregnancy
Pregnancy-Specific beta 1-Glycoproteins - genetics
Pregnancy. Parturition. Lactation
Promoter Regions, Genetic - genetics
Protein Binding
Regulatory Sequences, Nucleic Acid - genetics
Reproductive Biology
Sexual reproduction
Sp1 Transcription Factor - genetics
TATA Box less
Tissue specific
Transcription, Genetic - genetics
Transfection
Trophoblasts - metabolism
Vertebrates: reproduction
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Summary:Pregnancy-specific glycoprotein 5 gene (PSG-5) belongs to the human pregnancy-specific glycoprotein family, encoded by eleven highly similar and transcriptionally active genes. High levels of PSG biosynthesis are restricted to the placenta syncytiotrophoblast and are essential for the maintenance of normal gestation in mammalian species. We have investigated here the nature of the transcription factors that recognize the FP1 (–455/–433) and the CPE (–147/–140) regulatory sequences that significantly contribute to basal PSG-5 promoter activity. Both elements bear a similar GT-box motif; and DNA-protein complex formation, as well as promoter activity, is largely dependent on the integrity of these GT-box sequences. Gel shift, super gel shift and UV-crosslinking experiments clearly demonstrate that the ubiquitous specificity protein 1 (Sp1) is the major transcription factor involved in complex formation with both cis-acting elements in normal term placenta tissue and in PSG-non-expressing COS-7 cells. Furthermore, transfection experiments indicate that Sp1 activates PSG-5 promoter constructs. In addition, we show that Sp1 is indeed co-expressed with PSG genes in the syncytiotrophoblast cells, stressing its potential role in the in vivo regulation of PSG expression.
ISSN:0143-4004
1532-3102
DOI:10.1016/S0143-4004(03)00213-3