Steroids and adrenomedullin growth patterns in human ovarian cancer cells: estrogenic-regulation assay

Adrenomedullin is a multifunctional regulatory peptide with mitogenic and angiogenic capabilities. Objectives in this study were: (1) to describe the effects of adrenomedullin and anti-adrenomedullin antibodies on ovarian carcinoma cell proliferation, and (2) to assess the estradiol regulation of ad...

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Published in:Gynecologic oncology 2003-12, Vol.91 (3), p.651-656
Main Authors: Giacalone, Pierre-Ludovic, Daurés, J.P, Ouafik, L’Houcine, Martin, Pierre-Marie, Laffargue, François, Maudelonde, Thierry
Format: Article
Language:English
Subjects:
Adrenomedullin
Anti-adrenomedullin antibodies
Antibodies - immunology
Antibodies - pharmacology
Biological and medical sciences
Cancer
Cell Division - drug effects
Cell Division - physiology
Cell Line, Tumor
Estradiol
Estradiol - immunology
Estradiol - pharmacology
Estradiol - physiology
Female
Female genital diseases
Gynecology. Andrology. Obstetrics
Humans
Life Sciences
Medical sciences
Ovarian cancer
Ovarian Neoplasms - pathology
Peptides - genetics
Peptides - immunology
Peptides - pharmacology
Peptides - physiology
RNA, Messenger - biosynthesis
RNA, Messenger - genetics
Tumors
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Summary:Adrenomedullin is a multifunctional regulatory peptide with mitogenic and angiogenic capabilities. Objectives in this study were: (1) to describe the effects of adrenomedullin and anti-adrenomedullin antibodies on ovarian carcinoma cell proliferation, and (2) to assess the estradiol regulation of adrenomedullin metabolism. We assessed the effects of estradiol, adrenomedullin, and anti-adrenomedullin antibodies on cell growth in three human ovarian cell lines. RT-PCR was used to assess mRNA expression and Western blots to determine protein levels. Estradiol stimulates BG-1 and PEO4 cells growth but not PEO14 cells. Adrenomedullin mRNA expression and secretion were not under estrogen control. Adrenomedullin and anti-adrenomedullin antibodies had no growth effects in vitro. Adjunction of anti-adrenomedullin antibodies to estradiol-stimulated cells significantly inhibited their growth. Adrenomedullin metabolism is not under estradiol control. Anti-adrenomedullin antibodies display inhibitory effects on cells having high mitogenic activity. This opens the need for additional search toward in vivo specific immunotherapy.
ISSN:0090-8258
1095-6859
DOI:10.1016/S0090-8258(03)00279-8