Immunization with AgTRIO, a Protein in &ITAnopheles &ITSaliva, Contributes to Protection against &ITPlasmodium&IT Infection in Mice

Plasmodium infection begins with the bite of an anopheline mosquito, when sporozoites along with saliva are injected into a vertebrate host. The role of the host responses to mosquito saliva components in malaria remains unclear. We observed that antisera against Anopheles gambiae salivary glands pa...

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Bibliographic Details
Published in:Cell host & microbe 2018-04, Vol.23 (4)
Main Authors: Dragovic, Srdjan M., Agunbiade, Tolulope A., Freudzon, Marianna, Yang, Jing, Hastings, Andrew K., Schleicher, Tyler R., Zhou, Xia, Craft, Sam, Chuang, Yu-Min, Gonzalez, Floricel, Li, Youquan, Hrebikova, Gabriela, Tripathi, Abhai, Mlambo, Godfree, Almeras, Lionel, Ploss, Alexander, Dimopoulos, George, Fikrig, Erol
Format: Article
Language:English
Subjects:
Human health and pathology
Infectious diseases
Life Sciences
Online Access:Get full text
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Summary:Plasmodium infection begins with the bite of an anopheline mosquito, when sporozoites along with saliva are injected into a vertebrate host. The role of the host responses to mosquito saliva components in malaria remains unclear. We observed that antisera against Anopheles gambiae salivary glands partially protected mice from mosquito-borne Plasmodium infection. Specifically, antibodies to A. gambiae TRIO (AgTRIO), a mosquito salivary gland antigen, contributed to the protection. Mice administered AgTRIO antiserum showed lower Plasmodium liver burden and decreased parasitemia when exposed to infected mosquitoes. Active immunization with AgTRIO was also partially protective against Plasmodium berghei infection. A combination of AgTRIO antiserum and antibodies against Plasmodium circumsporozoite protein, a vaccine candidate, further decreased P. berghei infection. In humanized mice, AgTRIO antiserum afforded some protection against mosquito-transmitted Plasmodium falciparum. AgTRIO antiserum reduced the movement of sporozoites in the murine dermis. AgTRIO may serve as an arthropod-based target against Plasmodium to combat malaria.
ISSN:1931-3128
DOI:10.1016/j.chom.2018.03.008