Simultaneous UHPLC-UV analysis of hydroxychloroquine, minocycline and doxycycline from serum samples for the therapeutic drug monitoring of Q fever and Whipple’s disease

•Fast therapeutic drug monitoring method.•Simple method adapted to any laboratory.•First published monitoring method for Q fever and Whipple’s disease. A fast UHPLC-UV method was developed for the simultaneous analysis of Hydroxychloroquine, Minocycline and Doxycycline drugs from 100μL of human seru...

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Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2017-08, Vol.1060, p.166-172
Main Authors: Armstrong, Nicholas, Richez, Magali, Raoult, Didier, Chabriere, Eric
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - blood
Anti-Bacterial Agents - pharmacokinetics
Anti-Bacterial Agents - therapeutic use
Chromatography, High Pressure Liquid - methods
Doxycycline
Doxycycline - blood
Doxycycline - chemistry
Doxycycline - pharmacokinetics
Doxycycline - therapeutic use
Drug Monitoring - methods
Drug Stability
Human health and pathology
Humans
Hydroxychloroquine
Hydroxychloroquine - blood
Hydroxychloroquine - chemistry
Hydroxychloroquine - pharmacokinetics
Hydroxychloroquine - therapeutic use
Life Sciences
Limit of Detection
Linear Models
Minocycline
Minocycline - blood
Minocycline - pharmacokinetics
Minocycline - therapeutic use
Q fever
Q Fever - drug therapy
Reproducibility of Results
UHPLC
Whipple Disease - drug therapy
Whipple’s disease
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Summary:•Fast therapeutic drug monitoring method.•Simple method adapted to any laboratory.•First published monitoring method for Q fever and Whipple’s disease. A fast UHPLC-UV method was developed for the simultaneous analysis of Hydroxychloroquine, Minocycline and Doxycycline drugs from 100μL of human serum samples. Serum samples were extracted by liquid–liquid extraction and injected into a phenyl hexyl reverse phase column. Compounds were separated using a mobile phase linear gradient and monitored by UV detection at 343nm. Chloroquine and Oxytetracycline were used as internal standards. Lower and upper limits of quantifications, as well as the other levels of calibration, were validated with acceptable accuracy (
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2017.06.011