Dual control of ROS1‐mediated active DNA demethylation by DNA damage‐binding protein 2 (DDB2)

Summary By controlling gene expression, DNA methylation contributes to key regulatory processes during plant development. Genomic methylation patterns are dynamic and must be properly maintained and/or re‐established upon DNA replication and active removal, and therefore require sophisticated contro...

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Bibliographic Details
Published in:The Plant journal : for cell and molecular biology 2017-12, Vol.92 (6), p.1170-1181
Main Authors: Córdoba‐Cañero, Dolores, Cognat, Valérie, Ariza, Rafael R., Roldán Arjona, Teresa, Molinier, Jean
Format: Article
Language:English
Subjects:
Active control
active DNA demethylation
AGO4
Arabidopsis - genetics
Arabidopsis Proteins - genetics
Arabidopsis Proteins - metabolism
Arabidopsis thaliana
Argonaute Proteins - genetics
Argonaute Proteins - metabolism
Binding
Biochemistry, Molecular Biology
Botany
DDB2
Demethylation
Deoxyribonucleic acid
DNA
DNA biosynthesis
DNA Damage
DNA Demethylation
DNA Methylation
DNA repair
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
Endonuclease
Endonucleases - genetics
Endonucleases - metabolism
Enzymatic activity
Gene expression
Gene Expression Regulation, Plant
Intermediates
Life Sciences
Molecular biology
Nuclear Proteins - genetics
Nuclear Proteins - metabolism
Nucleotidases - genetics
Nucleotidases - metabolism
Proteins
ROS1
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Summary:Summary By controlling gene expression, DNA methylation contributes to key regulatory processes during plant development. Genomic methylation patterns are dynamic and must be properly maintained and/or re‐established upon DNA replication and active removal, and therefore require sophisticated control mechanisms. Here we identify direct interplay between the DNA repair factor DNA damage‐binding protein 2 (DDB2) and the ROS1‐mediated active DNA demethylation pathway in Arabidopsis thaliana. We show that DDB2 forms a complex with ROS1 and AGO4 and that they act at the ROS1 locus to modulate levels of DNA methylation and therefore ROS1 expression. We found that DDB2 represses enzymatic activity of ROS1. DNA demethylation intermediates generated by ROS1 are processed by the DNA 3′‐phosphatase ZDP and the apurinic/apyrimidinic endonuclease APE1L, and we also show that DDB2 interacts with both enzymes and stimulates their activities. Taken together, our results indicate that DDB2 acts as a critical regulator of ROS1‐mediated active DNA demethylation. Significance Statement DNA methylation landscape is the dynamic end result of methylation and demethylation processes. Our study unravels key aspects of a complex, dynamic interplay between DNA repair factors and components of the DNA methylation/demethylation machinery that contribute to shape the methylome.
ISSN:0960-7412
1365-313X
DOI:10.1111/tpj.13753