Structure of bacterial communities along a hydrocarbon contamination gradient in a coastal sediment

The bacterial diversity of a chronically oil-polluted retention basin sediment located in the Berre lagoon (Etang-de-Berre, France) was investigated. This study combines chemical and molecular approaches in order to define how the in situ petroleum hydrocarbon contamination level affects the bacteri...

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Bibliographic Details
Published in:FEMS microbiology ecology 2008-11, Vol.66 (2), p.295-305
Main Authors: Paissé, Sandrine, Coulon, Frédéric, Goñi-Urriza, Marisol, Peperzak, Louis, McGenity, Terry J, Duran, Robert
Format: Article
Language:English
Subjects:
16S rRNA gene library
Actinobacteria
Adaptation
Alkanes
Animal and plant ecology
Animal, plant and microbial ecology
Autosomal dominant inheritance
Bacteria
Bacteria - classification
Bacteria - genetics
bacterial diversity
Bacteriology
Biodegradation
Biological and medical sciences
Communities
Community composition
Community structure
Contamination
Content analysis
Cyanobacteria
Cyanophyta
DNA, Bacterial - analysis
DNA, Ribosomal - analysis
Ecology
Ecosystem
France
Fundamental and applied biological sciences. Psychology
Gene Library
Gene polymorphism
Genes, rRNA
Geologic Sediments - chemistry
Geologic Sediments - microbiology
hydrocarbon degradation
Hydrocarbons
Hydrocarbons - analysis
Lagoons
Life Sciences
Maintenance management
Microbial ecology
Microbiology
Microbiology and Parasitology
Molecular Sequence Data
Oil
Oil pollution
Organic chemistry
Petroleum
Petroleum hydrocarbons
Phylogeny
Polymerase Chain Reaction
Polymorphism
Polymorphism, Restriction Fragment Length
Pristane
Proteobacteria
Restriction fragment length polymorphism
Retention basins
RNA, Ribosomal, 16S
rRNA 16S
Sea water ecosystems
Sediment pollution
Sediments
Sequence Analysis, DNA
Settling basins
Sulfur
Synecology
T-RFLP
Various environments (extraatmospheric space, air, water)
Verrucomicrobium
Water Pollutants, Chemical - analysis
Water pollution
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Summary:The bacterial diversity of a chronically oil-polluted retention basin sediment located in the Berre lagoon (Etang-de-Berre, France) was investigated. This study combines chemical and molecular approaches in order to define how the in situ petroleum hydrocarbon contamination level affects the bacterial community structure of a subsurface sediment. Hydrocarbon content analysis clearly revealed a gradient of hydrocarbon contamination in both the water and the sediment following the basin periphery from the pollution input to the lagoon water. The nC17 and pristane concentrations suggested alkane biodegradation in the sediments. These results, combined with those of terminal-restriction fragment length polymorphism analysis of the 16S rRNA genes, indicated that bacterial community structure was obviously associated with the gradient of oil contamination. The analysis of bacterial community composition revealed dominance of bacteria related to the Proteobacteria phylum (Gamma-, Delta-, Alpha-, Epsilon- and Betaproteobacteria), Bacteroidetes and Verrucomicrobium groups and Spirochaetes, Actinobacteria and Cyanobacteria phyla. The adaptation of the bacterial community to oil contamination was not characterized by dominance of known oil-degrading bacteria, because a predominance of populations associated to the sulphur cycle was observed. The input station presented particular bacterial community composition associated with a low oil concentration in the sediment, indicating the adaptation of this community to the oil contamination.
ISSN:0168-6496
1574-6941
DOI:10.1111/j.1574-6941.2008.00589.x