Susceptibility of ten Haemonchus contortus isolates from different geographical origins towards acetone:water extracts of polyphenol-rich plants. Part 2: Infective L 3 larvae

This study explored the variation in susceptibility to acetone:water plant extracts between infective larvae (L ) of ten Haemonchus contortus isolates from different geographical origin. The L of 10 different isolates were exposed either to the acetone:water extract of a temperate plant (Onobrychis...

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Bibliographic Details
Published in:Veterinary parasitology 2017-06, Vol.240, p.11-16
Main Authors: Chan-Pérez, J I, Torres-Acosta, J F J, Sandoval-Castro, C A, Castañeda-Ramírez, G S, Vilarem, G, Mathieu, C, Hoste, H
Format: Article
Language:English
Subjects:
Acetone - chemistry
Agricultural sciences
Animals
Anthelmintics - chemistry
Anthelmintics - pharmacology
Goat Diseases - drug therapy
Goat Diseases - parasitology
Goats
Haemonchiasis - drug therapy
Haemonchiasis - veterinary
Haemonchus - drug effects
Larva - drug effects
Life Sciences
Plant Extracts - chemistry
Plant Extracts - pharmacology
Polyphenols - chemistry
Polyphenols - pharmacology
Water - chemistry
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Summary:This study explored the variation in susceptibility to acetone:water plant extracts between infective larvae (L ) of ten Haemonchus contortus isolates from different geographical origin. The L of 10 different isolates were exposed either to the acetone:water extract of a temperate plant (Onobrychis viciifolia) or a tropical plant (Acacia pennatula) and were evaluated with the larval exsheathment inhibition assay (LEIA). The L of each isolate were incubated with different concentrations of each extract (0, 25, 50, 100, 200, 400, 600, 800, 1000 and 1200μg/mL of phosphate buffered saline (PBS)). After incubation, the exsheathment process of L was induced using a solution with sodium hypochlorite (2%) and sodium chloride (16.5%). The proportion of exsheathed L was determined for each concentration at 0, 20, 40 and 60min. Effective concentrations 50% (EC ) and the corresponding 95% confidence intervals (95% CI) were calculated for every isolate with both extracts. Moreover, a resistance ratio (RR) was calculated for each extract to compare isolates, using the most susceptible isolate as the respective reference for each extract. To determine the role of polyphenols on the reported effect, a second set of incubations was made for each isolate and each extract, using the extracts at a concentration of 1200μg/mL PBS with or without polyvinylpolypyrrolidone (PVPP), a polyphenol blocking agent, and controls without extract. The ten different H. contortus isolates showed variation in susceptibility for each of the 2 extracts tested (P
ISSN:0304-4017
1873-2550
DOI:10.1016/j.vetpar.2017.04.023