Visualization of intracellular Ca2+ dynamics with simultaneous two-photon-excited fluorescence and third-harmonic generation microscopes

We present a promising development of third-harmonic generation (THG) coupled with two-photon excited fluorescence (TPEF) in laser scanning microscopy to study in vivo glial human cells. In this letter, intracellular Ca2+ dynamics in biological cells are shown. The THG microscopy allows visualizing...

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Bibliographic Details
Published in:Applied physics letters 2001-12, Vol.79 (24), p.4045-4047
Main Authors: Barille, R., Canioni, L., Rivet, S., Sarger, L., Vacher, P., Ducret, T.
Format: Article
Language:English
Subjects:
Physics
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Summary:We present a promising development of third-harmonic generation (THG) coupled with two-photon excited fluorescence (TPEF) in laser scanning microscopy to study in vivo glial human cells. In this letter, intracellular Ca2+ dynamics in biological cells are shown. The THG microscopy allows visualizing precisely the localization of the Ca2+ release whereas TPEF microscopy only gives functional mechanisms.
ISSN:0003-6951
1077-3118
DOI:10.1063/1.1425450