Evolutionary history of c-myc in teleosts and characterization of the duplicated c-myca genes in goldfish embryos

c‐Myc plays an important role during embryogenesis in mammals, but little is known about its function during embryonic development in teleosts. In addition, the evolutionary history of c‐myc gene in teleosts remains unclear, and depending on the species, a variable number of gene duplicates exist in...

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Bibliographic Details
Published in:Molecular reproduction and development 2012-02, Vol.79 (2), p.85-96
Main Authors: Marandel, Lucie, Labbe, Catherine, Bobe, Julien, Le Bail, Pierre-Yves
Format: Article
Language:English
Subjects:
Animals
Carassius auratus
Cyprinidae
Embryo, Nonmammalian
Evolution, Molecular
Fishes - embryology
Fishes - genetics
Freshwater
Gene Duplication - physiology
Gene Expression Regulation, Developmental
Genes, myc - genetics
Genome
Goldfish - embryology
Goldfish - genetics
Life Sciences
Molecular Sequence Data
Phylogeny
Pisces
Protein Isoforms - genetics
Protein Isoforms - metabolism
Reproductive Biology
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
Synteny
Zebrafish - genetics
Zebrafish - metabolism
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Summary:c‐Myc plays an important role during embryogenesis in mammals, but little is known about its function during embryonic development in teleosts. In addition, the evolutionary history of c‐myc gene in teleosts remains unclear, and depending on the species, a variable number of gene duplicates exist in teleosts. To gain new insight into c‐myc genes in teleosts, the present study was designed to clarify the evolutionary history of c‐myc gene(s) in teleosts and to subsequently characterize DNA methylation and early embryonic expression patterns in a cyprinid fish. Our results show that a duplication of c‐myc gene occurred before or around the teleost radiation, as a result of the teleost‐specific whole genome duplication giving rise to c‐myca and c‐mycb in teleosts and was followed by a loss of the c‐mycb gene in the Gasterosteiforms and Tetraodontiforms. Our data also demonstrate that both c‐myc genes previously identified in carp and goldfish are co‐orthologs of the zebrafish c‐myca. These results indicate the presence of additional c‐myca duplication in Cyprininae. We were able to identify differences between the expression patterns of the two goldfish c‐myca genes in oocytes and early embryos. These differences suggest a partial sub‐functionalization of c‐myca genes after duplication. Despite differences in transcription patterns, both of the c‐myca genes displayed similar DNA methylation patterns during early development and in gametes. Together, our results clarify the evolutionary history of the c‐myc gene in teleosts and provide new insight into the involvement of c‐myc in early embryonic development in cyprinids. Mol. Reprod. Dev. 79:85–96, 2012. © 2011 Wiley Periodicals, Inc.
ISSN:1040-452X
1098-2795
DOI:10.1002/mrd.22004