The duplicated rainbow trout (Oncorhynchus mykiss) T-box transcription factors 1, tbx1a and tbx1b, are up-regulated during testicular development

Tbx1 is a member of the T‐box transcription factor gene family involved in embryogenesis and organogenesis. Recently, within a pan‐genomic screen using rainbow trout (Oncorhynchus mykiss) cDNA microarrays, we identified a tbx1 homolog with testicular over‐expression during sex differentiation. Here,...

Full description

Saved in:
Bibliographic Details
Published in:Molecular reproduction and development 2011-03, Vol.78 (3), p.172-180
Main Authors: Yano, Ayaka, Nicol, Barbara, Guerin, Adele, Guiguen, Yann
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Aromatase Inhibitors - pharmacology
Biological and medical sciences
Computational Biology
DNA Primers - genetics
Estrogens - pharmacology
Freshwater
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Developmental - drug effects
Gene Expression Regulation, Developmental - physiology
Genes, Duplicate - genetics
In Situ Hybridization
Life Sciences
Male
Mammalian reproduction. General aspects
Molecular Sequence Data
Oncorhynchus mykiss
Oncorhynchus mykiss - growth & development
Phylogeny
Pisces
Polymerase Chain Reaction
Reproductive Biology
Salmonidae
Sequence Alignment
T-Box Domain Proteins - genetics
T-Box Domain Proteins - metabolism
Testis - growth & development
Testis - metabolism
Tretinoin - pharmacology
Vertebrates: reproduction
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Tbx1 is a member of the T‐box transcription factor gene family involved in embryogenesis and organogenesis. Recently, within a pan‐genomic screen using rainbow trout (Oncorhynchus mykiss) cDNA microarrays, we identified a tbx1 homolog with testicular over‐expression during sex differentiation. Here, we characterized two very similar rainbow trout tbx1 paralogs, tbx1a and tbx1b. In adult tissues, tbx1a expression is restricted to the gonads, with high expression in the testis, while tbx1b is more widely expressed in gonads, gills, brains, muscle, and skin. During gonadal differentiation, both genes are differentially expressed in favor of testis formation shortly after hatching. These genes are expressed in somatic cells surrounding germ cells of the differentiating testis, while no or only weak expression was observed in the differentiating ovary. tbx1a and tbx1b were also both down‐regulated in the differentiating testis during feminization with estrogens and up‐regulated in the differentiating ovary during masculinization with an aromatase inhibitor. These results suggest that tbx1a and tbx1b are probably involved in the regulation of testicular differentiation in rainbow trout. Since Tbx1 is known to interact with the retinoic acid (RA) signaling pathway, we also examined the effect of RA on the rainbow trout tbx1 expression pattern. Expression of tbx1a and tbx1b was down‐regulated in RA‐treated male gonads, suggesting that tbx1 interacts with the RA signaling pathway and thus could be involved in the control of rainbow trout gonadal differentiation. Mol. Reprod. Dev. 78:172–180, 2011. © 2011 Wiley‐Liss, Inc.
ISSN:1040-452X
1098-2795
1098-2795
DOI:10.1002/mrd.21279