Regulation of RhSUC2, a sucrose transporter, is correlated with the light control of bud burst in Rosa sp

ABSTRACT In roses, light is a central environmental factor controlling bud break and involves a stimulation of sugar metabolism. Very little is known about the role of sucrose transporters in the bud break process and its regulation by light. In this study, we show that sugar promotes rose bud break...

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Published in:Plant, cell and environment cell and environment, 2011-10, Vol.34 (10), p.1776-1789
Main Authors: HENRY, CLEMENCE, RABOT, AMELIE, LALOI, MARYSE, MORTREAU, ERIC, SIGOGNE, MONIQUE, LEDUC, NATHALIE, LEMOINE, RÉMI, SAKR, SOULAIMAN, VIAN, ALAIN, PELLESCHI‐TRAVIER, SANDRINE
Format: Article
Language:English
Subjects:
axillary bud
Biological and medical sciences
Biological Transport - radiation effects
buds
dominance
environmental factors
functional analysis
Fundamental and applied biological sciences. Psychology
imports
Life Sciences
Light
Membrane Transport Proteins - genetics
Membrane Transport Proteins - metabolism
Membrane Transport Proteins - radiation effects
Metabolism
Organs
photocontrol
photomorphogenesis
Plant Proteins - genetics
Plant Proteins - metabolism
Plant Proteins - radiation effects
Plant Stems - metabolism
Plant Stems - radiation effects
Rosa
Rosa - physiology
Rosa - radiation effects
Saccharomyces cerevisiae
sink strength
Sucrose - metabolism
Sucrose - radiation effects
sugar loading
Vegetal Biology
Yeasts
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Summary:ABSTRACT In roses, light is a central environmental factor controlling bud break and involves a stimulation of sugar metabolism. Very little is known about the role of sucrose transporters in the bud break process and its regulation by light. In this study, we show that sugar promotes rose bud break and that bud break is accompanied by an import of sucrose. Radio‐labelled sucrose accumulation is higher in buds exposed to light than to darkness and involves an active component. Several sucrose transporter (RhSUC1, 2, 3 and 4) transcripts are expressed in rose tissues, but RhSUC2 transcript level is the only one induced in buds exposed to light after removing the apical dominance. RhSUC2 is preferentially expressed in bursting buds and stems. Functional analyses in baker's yeast demonstrate that RhSUC2 encodes a sucrose/proton co‐transporter with a Km value of 2.99 mm at pH 4.5 and shows typical features of sucrose symporters. We therefore propose that bud break photocontrol partly depends upon the modulation of sucrose import into buds by RhSUC2. Previous works showed sugar implication in bud growth or transporter role in sink organs. But very little is known about the role of sucrose transporters in the bud break process and its regulation by environmental factors such as light. This article presents the first rose sucrose transporter isolation in bud and its functional validation. Results obtained showed that light controls bud burst in relation with the modulation of sucrose import into buds correlated with the induction of RhSUC2 expression.
ISSN:0140-7791
1365-3040
DOI:10.1111/j.1365-3040.2011.02374.x