Vacuolar biogenesis and aquaporin expression at early germination of broad bean seeds

A key event in seed germination is water uptake-mediated growth initiation in embryonic axes. Vicia faba var. minor (broad bean) seeds were used for studying cell growth, vacuolar biogenesis, expression and function of tonoplast water channel proteins (aquaporins) in embryonic axes during seed imbib...

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Bibliographic Details
Published in:Plant physiology and biochemistry 2014-09, Vol.82, p.123-132
Main Authors: Novikova, Galina V., Tournaire-Roux, Colette, Sinkevich, Irina A., Lityagina, Snejana V., Maurel, Christophe, Obroucheva, Natalie
Format: Article
Language:English
Subjects:
Aquaporins - metabolism
Cell elongation
Gene expression
Germination - physiology
Life Sciences
Plant Proteins - metabolism
Seed germination
Seeds - cytology
Seeds - physiology
Tonoplast aquaporins
Vacuole biogenesis
Vacuoles - metabolism
Vacuoles - physiology
Vegetal Biology
Vicia faba minor
Water - metabolism
Water transport
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Summary:A key event in seed germination is water uptake-mediated growth initiation in embryonic axes. Vicia faba var. minor (broad bean) seeds were used for studying cell growth, vacuolar biogenesis, expression and function of tonoplast water channel proteins (aquaporins) in embryonic axes during seed imbibition, radicle emergence and growth. Hypocotyl and radicle basal cells showed vacuole restoration from protein storage vacuoles, whereas de novo vacuole formation from provacuoles was observed in cells newly produced by root meristem. cDNA fragments of seven novel aquaporin isoforms including five Tonoplast Intrinsic Proteins (TIP) from three sub-types were amplified by PCR. The expression was probed using q-RT-PCR and when possible with isoform-specific antibodies. Decreased expression of TIP3s was associated to the transformation of protein storage vacuoles to vacuoles, whereas enhanced expression of a TIP2 homologue was closely linked to the fast cell elongation. Water channel functioning checked by inhibitory test with mercuric chloride showed closed water channels prior to growth initiation and active water transport into elongating cells. The data point to a crucial role of tonoplast aquaporins during germination, especially during growth of embryonic axes, due to accelerated water uptake and vacuole enlargement resulting in rapid cell elongation. •Vacuole biogenesis precedes cell elongation and differs in hypocotyl and radicle.•Hypogeal germination results from elongation of hypocotyl cells pushing radicle tip.•For the first time aquaporin genes were identified in Vicia faba embryonic axes.•Tonoplast aquaporin gene expression enhanced and changed with vacuole biogenesis.•Water channels are closed at radicle emergence but do operate in elongating cells.
ISSN:0981-9428
1873-2690
DOI:10.1016/j.plaphy.2014.05.014