Effect of Polyethylene Glycol in Pig Intestinal Allotransplantation Without Immunosuppression

Objectives We evaluated whether IGL-1, a graft preservation solution containing polyethylene glycol, improves the outcome of small bowel grafts in comparison to the University of Wisconsin (UW) solution in a pig allotransplantation model. Materials and Methods Seventeen pigs were randomly allocated...

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Published in:The Journal of surgical research 2012-08, Vol.176 (2), p.621-628
Main Authors: Yandza, Thierry, M.D., Ph.D, Tauc, Michel, Ph.D, Canioni, Danielle, M.D, Rogel-Gaillard, Claire, Ph.D, Bernard, Ghislaine, M.D., Ph.D, Bernard, Alain, M.D., Ph.D, Gugenheim, Jean, M.D., Ph.D
Format: Article
Language:English
Subjects:
acute cellular rejection
Acute Disease
Adenosine - pharmacology
Agricultural sciences
Allopurinol - pharmacology
Animals
Apoptosis - immunology
Caspase 3 - metabolism
Female
Glutathione - pharmacology
Graft Rejection - immunology
Graft Rejection - mortality
Graft Rejection - prevention & control
Graft Survival - drug effects
Graft Survival - immunology
IGL-1
Immunosuppression
Insulin - pharmacology
Intestinal Mucosa - immunology
Intestinal Mucosa - pathology
intestinal transplantation
Intestine, Small - immunology
Intestine, Small - pathology
Intestine, Small - transplantation
ischemia-reperfusion-injury
Life Sciences
Organ Preservation Solutions - pharmacology
pig
polyethylene glycol
Polyethylene Glycols - pharmacology
Raffinose - pharmacology
Reperfusion Injury - immunology
Reperfusion Injury - mortality
Reperfusion Injury - prevention & control
Surgery
Sus scrofa
Transplantation, Homologous
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Summary:Objectives We evaluated whether IGL-1, a graft preservation solution containing polyethylene glycol, improves the outcome of small bowel grafts in comparison to the University of Wisconsin (UW) solution in a pig allotransplantation model. Materials and Methods Seventeen pigs were randomly allocated to group 1 ( n = 10; intestinal allotransplantation with IGL-1) and group 2 ( n = 7; allotransplantation with UW). Pigs received no immunosuppression and were sacrificed on postoperative d (POD) 8. Intestinal specimens were obtained from the animal immediately before cold flushing (T0), 2 h after graft reperfusion (T1), and at sacrifice (T2). Results Survival rate to POD 8 was 50% in group 1 compared with 16% in group 2 ( P < 0.05); 62% of pigs in group 1 did not present any acute cellular rejection (ACR) compared to 16% in group 2 ( P < 0.05). Severe ACR rate was 25% in group 1 and 66% in group 2 ( P < 0.05). iNOS activity and intestinal caspase 3 levels increased significantly between T0 and T1 in group 1 compared to group 2 ( P < 0.05). Cell necrosis increased significantly between TO and T1 in group 2 compared with group 1 ( P < 0.05) whereas cell apoptosis was significantly higher at T1 compared with T0 in group 1 in comparison to group 2. Conclusions Our results show that IGL-1 improves intestinal graft viability as compared to UW solution, possibly by reducing graft immunogenicity and by favoring intestinal epithelial repair.
ISSN:0022-4804
1095-8673
DOI:10.1016/j.jss.2011.10.012