Detection of an estrogen derivative in two breast cancer cell lines using a single core multimodal probe for imaging (SCoMPI) imaged by a panel of luminescent and vibrational techniques

3-Methoxy-17α-ethynylestradiol or mestranol is a prodrug for ethynylestradiol and the estrogen component of some oral contraceptive formulations. We demonstrate here that a single core multimodal probe for imaging - SCoMPI - can be efficiently grafted onto mestranol allowing its tracking in two brea...

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Bibliographic Details
Published in:Analyst (London) 2013-10, Vol.138 (19), p.5627-5638
Main Authors: Clède, Sylvain, Lambert, François, Sandt, Christophe, Kascakova, Slavka, Unger, Miriam, Harté, Etienne, Plamont, Marie-Aude, Saint-Fort, Rénette, Deniset-Besseau, Ariane, Gueroui, Zoher, Hirschmugl, Carol, Lecomte, Sophie, Dazzi, Alexandre, Vessières, Anne, Policar, Clotilde
Format: Article
Language:English
Subjects:
Biochemistry, Molecular Biology
Bioengineering
Biotechnology
Breast
Breast Neoplasms - pathology
Cancer
Cell Line, Tumor
Confocal
Estrogens
Estrogens - analysis
Female
Fourier transforms
Humans
Imaging
Infrared spectroscopy
Life Sciences
Luminescent Measurements - methods
MCF-7 Cells
Multimodal Imaging - methods
Spectroscopy, Fourier Transform Infrared - methods
Vibration
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Summary:3-Methoxy-17α-ethynylestradiol or mestranol is a prodrug for ethynylestradiol and the estrogen component of some oral contraceptive formulations. We demonstrate here that a single core multimodal probe for imaging - SCoMPI - can be efficiently grafted onto mestranol allowing its tracking in two breast cancer cell lines, MDA-MB-231 and MCF-7 fixed cells. Correlative imaging studies based on luminescence (synchrotron UV spectromicroscopy, wide field and confocal fluorescence microscopies) and vibrational (AFMIR, synchrotron FTIR spectromicroscopy, synchrotron-based multiple beam FTIR imaging, confocal Raman microspectroscopy) spectroscopies were consistent with one another and showed a Golgi apparatus distribution of the SCoMPI-mestranol conjugate in both cell lines.
ISSN:0003-2654
1364-5528
DOI:10.1039/c3an00807j