The regulatory role of the tetrapeptide AcSDKP in skin and hair physiology and the prevention of ageing effects in these tissues - a potential cosmetic role

Synopsis The naturally occurring tetrapeptide acetyl‐N‐Ser‐Asp‐Lys‐Pro (AcSDKP) recognized as a potent angiogenic factor was shown recently to contribute to the repair of cutaneous injuries. In the current article, we report the ability of AcSDKP to exert a beneficial effect on normal healthy skin a...

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Bibliographic Details
Published in:International journal of cosmetic science 2013-06, Vol.35 (3), p.286-298
Main Authors: Hajem, N., Chapelle, A., Bignon, J., Pinault, A., Liu, J.-M., Salah-Mohellibi, N., Lati, E., Wdzieczak-Bakala, J.
Format: Article
Language:English
Subjects:
AcSDKP
Aging
Blotting, Western
Cell Line
Chemical Sciences
Cosmetics
Cosmetics industry
Hair - drug effects
Hair - physiology
Humans
Immunohistochemistry
Organic chemistry
Physiology
SIRT1
Skin Physiological Phenomena - drug effects
skin/hair ageing
stem cell
tight junctions
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Summary:Synopsis The naturally occurring tetrapeptide acetyl‐N‐Ser‐Asp‐Lys‐Pro (AcSDKP) recognized as a potent angiogenic factor was shown recently to contribute to the repair of cutaneous injuries. In the current article, we report the ability of AcSDKP to exert a beneficial effect on normal healthy skin and scalp and to compensate for the ageing process. In vitro AcSDKP at 10−11–10−7 M significantly stimulates the growth of human keratinocytes, fibroblasts and follicle dermal papilla cells. Moreover, it enhances the growth of human epidermal keratinocyte progenitor and stem cells as shown in a clonogenic survival assay. Topical treatment of ex vivo cultured skin explants with 10−5 M AcSDKP increases the thickness of the epidermis and upregulates the synthesis of keratins 14 and 19, fibronectin, collagen III and IV as well as the glycoaminoglycans (GAGs). In the ex vivo‐cultured hair follicles, AcSDKP promotes hair shaft elongation and induces morphological and molecular modifications matching the criteria of hair growth. Furthermore, AcSDKP at 10−11–10−7 M was shown to improve epidermal barrier, stimulating expression of three protein components of tight junctions (claudin‐1, occludin, ZO‐1) playing an important role in connecting neighbouring cells. This tetrapeptide exercises also activation of SIRT1 implicated in the control of cell longevity. Indeed, a two‐fold increase in the synthesis of SIRT1 by cultured keratinocytes was observed in the presence of 10−11–10−7 M AcSDKP. In conclusion, these findings provide convincing evidence of the regulatory role of AcSDKP in skin and hair physiology and suggest a cosmetic use of this natural tetrapeptide to prevent skin ageing and hair loss and to promote the cutaneous regeneration and hair growth. Résumé Le tétrapeptide acétyl‐N‐Ser‐Asp‐Lys‐Pro (AcSDKP) est connu comme un stimulateur de l'angiogenèse et joue un rôle bénéfique dans la réparation des lésions cutanées. Les résultats de cette étude ont permis de mettre en évidence des nouvelles propriétés biologiques de ce peptide vis‐à‐vis de la peau et du cuir chevelu. En effet, AcSDKP stimule significativement la croissance des kératinocytes, des fibroblastes et des cellules de la papille dermique du follicule pileux en culture ainsi que des cellules souches et des cellules progénitrices des kératinocytes de l'épiderme humain. Nous avons montré que l'application topique d'AcSDKP sur des explants de peau humaine maintenus en survie stimule significativement la producti
ISSN:0142-5463
1468-2494
1467-2494
DOI:10.1111/ics.12046