Co-distribution of annexin VI and actin in secretory ameloblasts and odontoblasts of rat incisor

Annexin VI and actin were detected by immunoblot analysis in the enamel- and dentin-related portions of dental tissues. Annexin VI was found mainly in the particulate fraction whereas actin was detected in both the soluble and particulate fractions. By immunoelectron microscopy, annexin VI antibodie...

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Bibliographic Details
Published in:Cell and tissue research 1991, Vol.263 (1), p.81-89
Main Authors: GOLDBERG, M, FEINBERG, J, LECOLLE, S, KAETZEL, M. A, RAINTEAU, D, LESSARD, J. L, DEDMAN, J. R, WEINMAN, S
Format: Article
Language:English
Subjects:
Actins - metabolism
Ameloblasts - metabolism
Ameloblasts - ultrastructure
Animals
Annexin A6
Biological and medical sciences
Calcium-Binding Proteins - metabolism
Chemical Sciences
Fundamental and applied biological sciences. Psychology
Immunoblotting
Microscopy, Immunoelectron
Mouth. Exocrine and endocrine salivary glands. Teeth. Esophagus
Odontoblasts - metabolism
Odontoblasts - ultrastructure
Organic chemistry
Rats
Rats, Inbred Strains
Tissue Distribution
Tooth - cytology
Tooth - metabolism
Vertebrates: digestive system
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Summary:Annexin VI and actin were detected by immunoblot analysis in the enamel- and dentin-related portions of dental tissues. Annexin VI was found mainly in the particulate fraction whereas actin was detected in both the soluble and particulate fractions. By immunoelectron microscopy, annexin VI antibodies conjugated with colloidal gold were seen to label the mitochondria, the cytosol and the nucleus of secretory ameloblasts and odontoblasts of rat incisor. In the processes of these cells, the plasmalemmal undercoat was labeled. Anti-actin antibodies labeled the desmosome-like junctions, the cytosol, and the mitochondria of the cell bodies. Extensive labeling was seen at the periphery of the Tomes' processes and odontoblast processes. These results suggest that annexin VI may play a role in Ca2(+)-regulation in the cell bodies, especially as a calcium receptor protein in the mitochondria. Moreover, annexin VI and actin seem to be co-distributed in secretory processes. Thus, these proteins might be both involved in exocytotic and endocytotic events.
ISSN:0302-766X
1432-0878
DOI:10.1007/bf00318402