The link between mitochondrial DNA hypervariable segment I heteroplasmy and ageing among genetically unrelated Latvians

Various studies have demonstrated that mitochondrial DNA (mtDNA) heteroplasmy tends to increase with age and that the observed frequency of heteroplasmy among populations mostly depends on the way it is measured. Therefore, we investigated age-related association on the presence of mtDNA heteroplasm...

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Bibliographic Details
Published in:Experimental gerontology 2011-07, Vol.46 (7), p.560-568
Main Authors: Pliss, Liana, Brakmanis, Andis, Ranka, Renate, Elferts, Didzis, Krumina, Astrida, Baumanis, Viesturs
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Aged, 80 and over
Ageing
Aging - genetics
Denaturing Gradient Gel Electrophoresis
Detection of mtDNA heteroplasmy
DNA, Mitochondrial - chemistry
DNA, Mitochondrial - physiology
Female
Geriatry and gerontology
Heteroplasmy
Human health and pathology
Humans
Latvia
Latvians
Life Sciences
Male
Middle Aged
mtDNA
Mutation
Sequence Analysis, DNA
Young Adult
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Summary:Various studies have demonstrated that mitochondrial DNA (mtDNA) heteroplasmy tends to increase with age and that the observed frequency of heteroplasmy among populations mostly depends on the way it is measured. Therefore, we investigated age-related association on the presence of mtDNA heteroplasmy within the hypervariable segment 1 (HVS-I) in a selected study group. The study group consisted of 300 maternally unrelated Latvians ranging in age from 18 to over 90 years. To determine the optimal method for mtDNA heteroplasmy detection, three approaches were used: (i) SURVEYOR Mutation Detection Kit, (ii) sequencing and (iii) denaturing gradient-gel electrophoresis (DGGE). Among the studied individuals, 30.3% were found to be heteroplasmic. The distribution of heteroplasmy statistically significantly increased with individuals' age (17%; 95% confidence interval [CI] 0.095–0.244 in the 18–40 year age group vs. 39%; [CI] 0.294–0.487 in the > 90 year age group). Heteroplasmy occurred in a total of 21 different positions within HVS-I, and was the most frequent at fast-mutated positions 16189, 16304 and 16311. The results indicate that heteroplasmy in HVS-I is relatively common and occurs in a broad spectrum of sites. The above is supported by evidence to eventual increase of the probability of heteroplasmy with age due to specific mitochondrial haplogroup background. ► The results indicate that heteroplasmy in HVS-I is relatively common. ► A strong association is found between the mtDNA heteroplasmy and ageing. ► The DGGE and SURVEYOR Mutation Detection assay exhibited a lower limit of detection.
ISSN:0531-5565
1873-6815
DOI:10.1016/j.exger.2011.02.016