MALDI Imaging and Structural Analysis of Rat Brain Lipid Negative Ions with 9-Aminoacridine Matrix

Mass spectrometry imaging is of growing interest for chemical mapping of lipids at the surface of tissue sections. Many efforts have been devoted to optimize matrix choice and deposition technique for positive ion mode analyses. The identification of lipid species desorbed from tissue sections in th...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2012-03, Vol.84 (5), p.2164-2171
Main Authors: Cerruti, Christopher D, Benabdellah, Farida, Laprévote, Olivier, Touboul, David, Brunelle, Alain
Format: Article
Language:English
Subjects:
Aminacrine - chemistry
Analytical chemistry
Animals
Brain
Brain - metabolism
Chemical Sciences
Chemistry
Exact sciences and technology
Fatty acids
Ions - chemistry
Lipids
Lipids - analysis
Mass spectrometry
Nonnative species
Organic chemistry
Rats
Rodents
Signal-To-Noise Ratio
Spectrometric and optical methods
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Tandem Mass Spectrometry
Tissues
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Summary:Mass spectrometry imaging is of growing interest for chemical mapping of lipids at the surface of tissue sections. Many efforts have been devoted to optimize matrix choice and deposition technique for positive ion mode analyses. The identification of lipid species desorbed from tissue sections in the negative mode can be significantly improved by using 9-aminoacridine together with a robust deposition method, yielding a superior signal-to-noise ratio and thus a better contrast for the ion images in comparison to classical matrices such as α-cyano-4-hydroxycinnamic acid, 2,5-dihydroxybenzoic acid, or 2,4,6-trihydroxyacetophenone. Twenty-eight different lipid species (phosphatidic acids, phosphatidylethanolamines, phosphatidylserines, phosphatidylglycerols, phosphatidylinositols, phosphatidylinositol-phosphates, and sulfatides) were scrutinized on rat brain tissue sections, and systematic MS/MS studies were conducted. It was possible to identify isobaric species differing by their fatty acid chains thanks to the improved sensitivity.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac2025317